This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1aj9
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1aj9.gif|left|200px]] | [[Image:1aj9.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1aj9", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | | | + | {{STRUCTURE_1aj9| PDB=1aj9 | SCENE= }} |
| - | | | + | |
| - | + | ||
| - | }} | + | |
'''R-STATE HUMAN CARBONMONOXYHEMOGLOBIN ALPHA-A53S''' | '''R-STATE HUMAN CARBONMONOXYHEMOGLOBIN ALPHA-A53S''' | ||
| Line 29: | Line 26: | ||
[[Category: Ji, X.]] | [[Category: Ji, X.]] | ||
[[Category: Vasquez, G B.]] | [[Category: Vasquez, G B.]] | ||
| - | [[Category: | + | [[Category: Alpha-a53]] |
| - | [[Category: | + | [[Category: Carbonmonoxide]] |
| - | [[Category: | + | [[Category: Carbonmonoxy]] |
| - | [[Category: | + | [[Category: Carbonmonoxyhemoglobin]] |
| - | [[Category: | + | [[Category: Carboxyhemoglobin]] |
| - | [[Category: | + | [[Category: Hemoglobin]] |
| - | [[Category: | + | [[Category: Mutant]] |
| - | [[Category: | + | [[Category: Oxygen transport]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:20:36 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:20, 2 May 2008
R-STATE HUMAN CARBONMONOXYHEMOGLOBIN ALPHA-A53S
Overview
The three-dimensional structure and associated solvent of human carboxyhemoglobin at 2.2 A resolution are compared with other R-state and T-state human hemoglobin structures. The crystal form is isomorphous with that of the 2.7 A structure of carboxyhemoglobin reported earlier [Baldwin (1980). J. Mol. Biol. 136, 103-128], whose coordinates were used as a starting model, and with the 2.2 A structure described in an earlier report [Derewenda et al. (1990). J. Mol. Biol. 211, 515-519]. During the course of the refinement, a natural mutation of the alpha-subunit, A53S, was discovered that forms a new crystal contact through a bridging water molecule. The protein structure shows a significant difference between the alpha and beta heme geometries, with Fe-C-O angles of 125 and 162 degrees, respectively. The carboxyhemoglobin is compared with other fully ligated R-state human hemoglobins [Baldwin (1980). J. Mol. Biol. 136, 103-128; Shaanan (1983). J. Mol. Biol. 195, 419-422] with the R2-state hemoglobin [Silva et al. (1992). J. Biol. Chem. 267, 17248-17256] and with T-state deoxyhemoglobin [Fronticelli et al. (1994). J. Biol. Chem. 269, 23965-23969]. The structure is similar to the earlier reported R-state structures, but there are differences in many side-chain conformations, the associated water structure and the presence and the position of a phosphate ion. The quaternary changes between the R-state carboxyhemoglobin and the R2-state and T-state structures are in general consistent with those reported in the earlier structures. The location of 238 water molecules and a phosphate ion in the carboxyhemoglobin structure allows the first comparison of the solvent structures of the R-state and T-state structures. Distinctive hydration patterns for each of the quaternary structures are observed, but a number of conserved water molecule binding sites are found that are independent of the conformational state of the protein.
About this Structure
1AJ9 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Human carboxyhemoglobin at 2.2 A resolution: structure and solvent comparisons of R-state, R2-state and T-state hemoglobins., Vasquez GB, Ji X, Fronticelli C, Gilliland GL, Acta Crystallogr D Biol Crystallogr. 1998 May 1;54(Pt 3):355-66. PMID:9761903 Page seeded by OCA on Fri May 2 10:20:36 2008
