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2kbt

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 ==Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method==
-<StructureSection load='2kbt' size='340' side='right' caption='[[2kbt]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2kbt' size='340' side='right'caption='[[2kbt]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2kbt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strsg Strsg]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KBT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KBT FirstGlance]. <br>
@@ Line 31: / Line 31: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Strsg]]
 [[Category: Inagaki, F]]

Revision as of 09:34, 26 February 2020

Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method

spinqualitylabelsall models

PDB ID 2kbt

Show:Asymmetric Unit Biological Assembly

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Structural highlights

2kbt is a 1 chain structure with sequence from Strsg. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[VAV_MOUSE] Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Sample solubility is essential for structural studies of proteins by solution NMR. Attachment of a solubility enhancement tag, such as GB1, MBP and thioredoxin, to a target protein has been used for this purpose. However, signal overlap of the tag with the target protein often made the spectral analysis difficult. Here we report a sortase-mediated protein ligation method to eliminate NMR signals arising from the tag by preparing the isotopically labeled target protein attached with the non-labeled GB1 tag at the C-terminus.

Attachment of an NMR-invisible solubility enhancement tag using a sortase-mediated protein ligation method.,Kobashigawa Y, Kumeta H, Ogura K, Inagaki F J Biomol NMR. 2009 Jan 13. PMID:19140010^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kobashigawa Y, Kumeta H, Ogura K, Inagaki F. Attachment of an NMR-invisible solubility enhancement tag using a sortase-mediated protein ligation method. J Biomol NMR. 2009 Jan 13. PMID:19140010 doi:10.1007/s10858-008-9296-5

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

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2kbt

From Proteopedia

Revision as of 09:34, 26 February 2020

Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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