1akz
From Proteopedia
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[[Image:1akz.gif|left|200px]] | [[Image:1akz.gif|left|200px]] | ||
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| - | + | {{STRUCTURE_1akz| PDB=1akz | SCENE= }} | |
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'''HUMAN URACIL-DNA GLYCOSYLASE''' | '''HUMAN URACIL-DNA GLYCOSYLASE''' | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Tainer, C D.M J.A.]] | [[Category: Tainer, C D.M J.A.]] | ||
| - | [[Category: | + | [[Category: Alpha/ beta protein]] |
| - | [[Category: | + | [[Category: Dna repair]] |
| - | [[Category: | + | [[Category: Glycosidase]] |
| - | [[Category: | + | [[Category: Glycosylase]] |
| - | [[Category: | + | [[Category: Uracil removal from dna]] |
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:24, 2 May 2008
HUMAN URACIL-DNA GLYCOSYLASE
Overview
Crystal structures of the DNA repair enzyme human uracil-DNA glycosylase (UDG), combined with mutational analysis, reveal the structural basis for the specificity of the enzyme. Within the classic alpha/beta fold of UDG, sequence-conserved residues form a positively charged, active-site groove the width of duplex DNA, at the C-terminal edge of the central four-stranded parallel beta sheet. In the UDG-6-aminouracil complex, uracil binds at the base of the groove within a rigid preformed pocket that confers selectivity for uracil over other bases by shape complementary and by main chain and Asn-204 side chain hydrogen bonds. Main chain nitrogen atoms are positioned to stabilize the oxyanion intermediate generated by His-268 acting via nucleophilic attack or general base mechanisms. Specific binding of uracil flipped out from a DNA duplex provides a structural mechanism for damaged base recognition.
About this Structure
1AKZ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure and mutational analysis of human uracil-DNA glycosylase: structural basis for specificity and catalysis., Mol CD, Arvai AS, Slupphaug G, Kavli B, Alseth I, Krokan HE, Tainer JA, Cell. 1995 Mar 24;80(6):869-78. PMID:7697717 Page seeded by OCA on Fri May 2 10:24:23 2008
