5h66

From Proteopedia

(Difference between revisions)

Revision as of 10:12, 26 February 2020

Crystal structure of the Bacillus subtilis SMC head domain complexed with the cognate ScpA C-terminal domain

Structural highlights

5h66 is a 3 chain structure with sequence from Bacsu. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5h67, 5h68, 5h69, 3w6j, 3w6k
Gene:	smc, ylqA, BSU15940 (BACSU), scpA, ypuG, BSU23220 (BACSU)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SMC_BACSU] Required for chromosome condensation and partitioning.^[1] ^[2] [SCPA_BACSU] Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpB that pull DNA away from mid-cell into both cell halves.[HAMAP-Rule:MF_01805]

Publication Abstract from PubMed

The SMC-ScpAB complex plays a crucial role in chromosome organization and segregation in many bacteria. It is composed of a V-shaped SMC dimer and an ScpAB subcomplex that bridges the two Structural Maintenance of Chromosomes (SMC) head domains. Despite its functional significance, the mechanistic details of SMC-ScpAB remain obscure. Here we provide evidence that ATP-dependent head-head engagement induces a lever movement of the SMC neck region, which might help to separate juxtaposed coiled-coil arms. Binding of the ScpA N-terminal domain (NTD) to the SMC neck region is negatively regulated by the ScpB C-terminal domain. Mutations in the ScpA NTD compromise this regulation and profoundly affect the overall shape of the complex. The SMC hinge domain is structurally relaxed when free from coiled-coil juxtaposition. Taken together, we propose that the structural parts of SMC-ScpAB are subjected to the balance between constraint and relaxation, cooperating to modulate dynamic conformational changes of the whole complex.

Overall Shapes of the SMC-ScpAB Complex Are Determined by Balance between Constraint and Relaxation of Its Structural Parts.,Kamada K, Su'etsugu M, Takada H, Miyata M, Hirano T Structure. 2017 Apr 4;25(4):603-616.e4. doi: 10.1016/j.str.2017.02.008. Epub 2017, Mar 9. PMID:28286005^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Britton RA, Lin DC, Grossman AD. Characterization of a prokaryotic SMC protein involved in chromosome partitioning. Genes Dev. 1998 May 1;12(9):1254-9. PMID:9573042
↑ Moriya S, Tsujikawa E, Hassan AK, Asai K, Kodama T, Ogasawara N. A Bacillus subtilis gene-encoding protein homologous to eukaryotic SMC motor protein is necessary for chromosome partition. Mol Microbiol. 1998 Jul;29(1):179-87. PMID:9701812
↑ Kamada K, Su'etsugu M, Takada H, Miyata M, Hirano T. Overall Shapes of the SMC-ScpAB Complex Are Determined by Balance between Constraint and Relaxation of Its Structural Parts. Structure. 2017 Apr 4;25(4):603-616.e4. doi: 10.1016/j.str.2017.02.008. Epub 2017, Mar 9. PMID:28286005 doi:http://dx.doi.org/10.1016/j.str.2017.02.008

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5h66"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Bacillus subtilis SMC head domain complexed with the cognate ScpA C-terminal domain==
-<StructureSection load='5h66' size='340' side='right' caption='[[5h66]], [[Resolution|resolution]] 1.82&Aring;' scene=''>
+<StructureSection load='5h66' size='340' side='right'caption='[[5h66]], [[Resolution|resolution]] 1.82&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5h66]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H66 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H66 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5h66]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H66 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H66 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5h67|5h67]], [[5h68|5h68]], [[5h69|5h69]], [[3w6j|3w6j]], [[3w6k|3w6k]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">smc, ylqA, BSU15940 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU]), scpA, ypuG, BSU23220 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h66 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h66 OCA], [http://pdbe.org/5h66 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h66 RCSB], [http://www.ebi.ac.uk/pdbsum/5h66 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h66 ProSAT]</span></td></tr>
 </table>
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Bacsu]]
+[[Category: Large Structures]]
 [[Category: Hirano, T]]
 [[Category: Kamada, K]]
 [[Category: Dna binding protein-cell cycle complex]]
 [[Category: Smc protein]]

5h66

From Proteopedia

Revision as of 10:12, 26 February 2020

Crystal structure of the Bacillus subtilis SMC head domain complexed with the cognate ScpA C-terminal domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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