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4uqf
From Proteopedia
(Difference between revisions)
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==CRYSTAL STRUCTURE OF LISTERIA MONOCYTOGENES GTP CYCLOHYDROLASE I== | ==CRYSTAL STRUCTURE OF LISTERIA MONOCYTOGENES GTP CYCLOHYDROLASE I== | ||
| - | <StructureSection load='4uqf' size='340' side='right' caption='[[4uqf]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='4uqf' size='340' side='right'caption='[[4uqf]], [[Resolution|resolution]] 2.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4uqf]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_monocytogenes_hominis"_nyfeldt_1932 "bacterium monocytogenes hominis" nyfeldt 1932]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UQF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UQF FirstGlance]. <br> | <table><tr><td colspan='2'>[[4uqf]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_monocytogenes_hominis"_nyfeldt_1932 "bacterium monocytogenes hominis" nyfeldt 1932]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UQF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UQF FirstGlance]. <br> | ||
| - | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/GTP_cyclohydrolase_I GTP cyclohydrolase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.16 3.5.4.16] </span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">folE, B4Y57_10615, D3B94_12135, FORC68_1988 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1639 "Bacterium monocytogenes hominis" Nyfeldt 1932])</td></tr> |
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/GTP_cyclohydrolase_I GTP cyclohydrolase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.16 3.5.4.16] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uqf OCA], [http://pdbe.org/4uqf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uqf RCSB], [http://www.ebi.ac.uk/pdbsum/4uqf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4uqf ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uqf OCA], [http://pdbe.org/4uqf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uqf RCSB], [http://www.ebi.ac.uk/pdbsum/4uqf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4uqf ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A putative open reading frame encoding GTP cyclohydrolase I from Listeria monocytogenes was expressed in a recombinant Escherichia coli strain. The recombinant protein was purified and was confirmed to convert GTP to dihydroneopterin triphosphate (Km = 53 microM; vmax = 180 nmol mg(-1) min(-1)). The protein was crystallized from 1.3 M sodium citrate pH 7.3 and the crystal structure was solved at a resolution of 2.4 A (Rfree = 0.226) by molecular replacement using human GTP cyclohydrolase I as a template. The protein is a D5-symmetric decamer with ten topologically equivalent active sites. Screening a small library of about 9000 compounds afforded several inhibitors with IC50 values in the low-micromolar range. Several inhibitors had significant selectivity with regard to human GTP cyclohydrolase I. Hence, GTP cyclohydrolase I may be a potential target for novel drugs directed at microbial infections, including listeriosis, a rare disease with high mortality. | ||
| + | |||
| + | Structure of GTP cyclohydrolase I from Listeria monocytogenes, a potential anti-infective drug target.,Schussler S, Haase I, Perbandt M, Illarionov B, Siemens A, Richter K, Bacher A, Fischer M, Grawert T Acta Crystallogr F Struct Biol Commun. 2019 Sep 1;75(Pt 9):586-592. doi:, 10.1107/S2053230X19010902. Epub 2019 Aug 30. PMID:31475925<ref>PMID:31475925</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 4uqf" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
| - | *[[Cyclohydrolase|Cyclohydrolase]] | + | *[[Cyclohydrolase 3D structures|Cyclohydrolase 3D structures]] |
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bacterium monocytogenes hominis nyfeldt 1932]] | [[Category: Bacterium monocytogenes hominis nyfeldt 1932]] | ||
[[Category: GTP cyclohydrolase I]] | [[Category: GTP cyclohydrolase I]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Fischer, M]] | [[Category: Fischer, M]] | ||
[[Category: Graewert, T]] | [[Category: Graewert, T]] | ||
Revision as of 10:43, 26 February 2020
CRYSTAL STRUCTURE OF LISTERIA MONOCYTOGENES GTP CYCLOHYDROLASE I
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