6l6w
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The structure of ScoE with intermediate== |
| + | <StructureSection load='6l6w' size='340' side='right'caption='[[6l6w]], [[Resolution|resolution]] 2.17Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6l6w]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L6W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L6W FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=E79:(3R)-3-[[(1R)-1,2-bis(oxidanyl)-2-oxidanylidene-ethyl]amino]butanoic+acid'>E79</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l6w OCA], [http://pdbe.org/6l6w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l6w RCSB], [http://www.ebi.ac.uk/pdbsum/6l6w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l6w ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | N-alkylisonitrile, a precursor to isonitrile-containing lipopeptides, is biosynthesized via decarboxylation-assisted -N identical withC group (isonitrile) formation by using N-alkylglycine as the substrate. This reaction is catalyzed by iron(II) and 2-oxoglutarate (Fe/2OG) dependent enzymes. Distinct from typical oxygenation or halogenation reactions catalyzed by this class of enzymes, installation of the isonitrile group represents a novel reaction type for Fe/2OG enzymes that involves a four-electron oxidative process. Here we report the plausible mechanism of three Fe/2OG enzymes, Sav607, ScoE and SfaA, catalyzed isonitrile formation. The X-ray structures of iron loaded ScoE in complex with its substrate and the intermediate, along with biochemical and biophysical data reveal that -N identical withC bond formation involves two cycles of Fe/2OG enzyme catalysis. The reaction starts with an Fe(IV)-oxo catalyzed hydroxylation. It is likely followed by decarboxylation-assisted desaturation to complete isonitrile installation. | ||
| - | + | Pathway from N-alkylglycine to alkylisonitrile catalyzed by iron(II) and 2-oxoglutarate dependent oxygenases.,Chang WC, Chen TY, Chen J, Tang Y, Zhou J, Guo Y Angew Chem Int Ed Engl. 2020 Feb 19. doi: 10.1002/anie.201914896. PMID:32074393<ref>PMID:32074393</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6l6w" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Chang, W]] | ||
| + | [[Category: Chen, J]] | ||
| + | [[Category: Chen, T Y]] | ||
| + | [[Category: Zhou, J]] | ||
| + | [[Category: Oxidoreductase]] | ||
| + | [[Category: Scoe]] | ||
Revision as of 06:42, 4 March 2020
The structure of ScoE with intermediate
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Categories: Large Structures | Chang, W | Chen, J | Chen, T Y | Zhou, J | Oxidoreductase | Scoe
