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5kep

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Revision as of 04:33, 5 March 2020

High resolution cryo-EM maps of Human Papillomavirus 16 reveal L2 location and heparin-induced conformational changes

5kep, resolution 4.30Å

Structural highlights

5kep is a 6 chain structure with sequence from Hpv16. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5keq, 5key, 5kgb
Gene:	L1 (HPV16)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[VL1_HPV16] Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.[HAMAP-Rule:MF_04002]^[1] ^[2] ^[3]

Publication Abstract from PubMed

Human papillomavirus (HPV) is a significant health burden and leading cause of virus-induced cancers. The current commercial vaccines are genotype specific and provide little therapeutic benefit to patients with existing HPV infections. Host entry mechanisms represent an excellent target for alternative therapeutics, but HPV receptor use, the details of cell attachment, and host entry are inadequately understood. Here we present near-atomic resolution structures of the HPV16 capsid and HPV16 in complex with heparin, both determined from cryoelectron micrographs collected with direct electron detection technology. The structures clarify details of capsid architecture for the first time, including variation in L1 major capsid protein conformation and putative location of L2 minor protein. Heparin binds specifically around the capsid icosahedral vertices and may recapitulate the earliest stage of infection, providing a framework for continuing biochemical, genetic, and biophysical studies.

Cryoelectron Microscopy Maps of Human Papillomavirus 16 Reveal L2 Densities and Heparin Binding Site.,Guan J, Bywaters SM, Brendle SA, Ashley RE, Makhov AM, Conway JF, Christensen ND, Hafenstein S Structure. 2016 Dec 31. pii: S0969-2126(16)30391-4. doi:, 10.1016/j.str.2016.12.001. PMID:28065506^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bousarghin L, Touze A, Sizaret PY, Coursaget P. Human papillomavirus types 16, 31, and 58 use different endocytosis pathways to enter cells. J Virol. 2003 Mar;77(6):3846-50. PMID:12610160
↑ Surviladze Z, Sterkand RT, Ozbun MA. Interaction of human papillomavirus type 16 particles with heparan sulfate and syndecan-1 molecules in the keratinocyte extracellular matrix plays an active role in infection. J Gen Virol. 2015 Aug;96(8):2232-41. doi: 10.1099/vir.0.000147. PMID:26289843 doi:http://dx.doi.org/10.1099/vir.0.000147
↑ Heino P, Dillner J, Schwartz S. Human papillomavirus type 16 capsid proteins produced from recombinant Semliki Forest virus assemble into virus-like particles. Virology. 1995 Dec 20;214(2):349-59. PMID:8553535 doi:http://dx.doi.org/10.1006/viro.1995.0044
↑ Guan J, Bywaters SM, Brendle SA, Ashley RE, Makhov AM, Conway JF, Christensen ND, Hafenstein S. Cryoelectron Microscopy Maps of Human Papillomavirus 16 Reveal L2 Densities and Heparin Binding Site. Structure. 2016 Dec 31. pii: S0969-2126(16)30391-4. doi:, 10.1016/j.str.2016.12.001. PMID:28065506 doi:http://dx.doi.org/10.1016/j.str.2016.12.001

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5kep"

@@ Line 1: / Line 1: @@
 ==High resolution cryo-EM maps of Human Papillomavirus 16 reveal L2 location and heparin-induced conformational changes==
-<StructureSection load='5kep' size='340' side='right' caption='[[5kep]], [[Resolution|resolution]] 4.30&Aring;' scene=''>
+<SX load='5kep' size='340' side='right' viewer='molstar' caption='[[5kep]], [[Resolution|resolution]] 4.30&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[5kep]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Hpv16 Hpv16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KEP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KEP FirstGlance]. <br>
@@ Line 19: / Line 19: @@
 </div>
 <div class="pdbe-citations 5kep" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Major capsid protein L1|Major capsid protein L1]]
 == References ==
 <references/>
 __TOC__
-</StructureSection>
+</SX>
 [[Category: Hpv16]]
+[[Category: Large Structures]]
 [[Category: Ashley, R E]]
 [[Category: Brendle, S A]]

5kep

From Proteopedia

Revision as of 04:33, 5 March 2020

High resolution cryo-EM maps of Human Papillomavirus 16 reveal L2 location and heparin-induced conformational changes

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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