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5vj6

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Revision as of 19:52, 6 March 2020

BG505 SOSIP.664 in complex with broadly neutralizing antibodies PG9 and 8ANC195

5vj6, resolution 11.50Å

Structural highlights

5vj6 is a 14 chain structure with sequence from 9hiv1 and Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	env (9HIV1), IGL@ (HUMAN)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[Q2N0S6_9HIV1] The envelope glyprotein gp160 precursor down-modulates cell surface CD4 antigen by interacting with it in the endoplasmic reticulum and blocking its transport to the cell surface (By similarity).[RuleBase:RU004292][SAAS:SAAS000328_004_020447] The gp120-gp41 heterodimer allows rapid transcytosis of the virus through CD4 negative cells such as simple epithelial monolayers of the intestinal, rectal and endocervical epithelial barriers. Both gp120 and gp41 specifically recognize glycosphingolipids galactosyl-ceramide (GalCer) or 3' sulfo-galactosyl-ceramide (GalS) present in the lipid rafts structures of epithelial cells. Binding to these alternative receptors allows the rapid transcytosis of the virus through the epithelial cells. This transcytotic vesicle-mediated transport of virions from the apical side to the basolateral side of the epithelial cells does not involve infection of the cells themselves (By similarity).[SAAS:SAAS000328_004_240990]

Publication Abstract from PubMed

The HIV-1 envelope (Env) glycoprotein binds to host cell receptors to mediate membrane fusion. The prefusion Env trimer is stabilized by V1V2 loops that interact at the trimer apex. Broadly neutralizing antibodies (bNAbs) against V1V2 loops, exemplified by PG9, bind asymmetrically as a single Fab to the apex of the symmetric Env trimer using a protruding CDRH3 to penetrate the Env glycan shield. Here we characterized a distinct mode of V1V2 epitope recognition by the new bNAb BG1 in which two Fabs bind asymmetrically per Env trimer using a compact CDRH3. Comparisons between cryo-EM structures of Env trimer complexed with BG1 (6.2 A resolution) and PG9 (11.5 A resolution) revealed a new V1V2-targeting strategy by BG1. Analyses of the EM structures provided information relevant to vaccine design including molecular details for different modes of asymmetric recognition of Env trimer and a binding model for BG1 recognition of V1V2 involving glycan flexibility.

Asymmetric recognition of HIV-1 Envelope trimer by V1V2 loop-targeting antibodies.,Wang H, Gristick HB, Scharf L, West AP, Galimidi RP, Seaman MS, Freund NT, Nussenzweig MC, Bjorkman PJ Elife. 2017 May 26;6. pii: e27389. doi: 10.7554/eLife.27389. PMID:28548638^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wang H, Gristick HB, Scharf L, West AP, Galimidi RP, Seaman MS, Freund NT, Nussenzweig MC, Bjorkman PJ. Asymmetric recognition of HIV-1 Envelope trimer by V1V2 loop-targeting antibodies. Elife. 2017 May 26;6. pii: e27389. doi: 10.7554/eLife.27389. PMID:28548638 doi:http://dx.doi.org/10.7554/eLife.27389

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5vj6"

@@ Line 1: / Line 1: @@
 ==BG505 SOSIP.664 in complex with broadly neutralizing antibodies PG9 and 8ANC195==
-<StructureSection load='5vj6' size='340' side='right' caption='[[5vj6]], [[Resolution|resolution]] 11.50&Aring;' scene=''>
+<SX load='5vj6' size='340' side='right' viewer='molstar' caption='[[5vj6]], [[Resolution|resolution]] 11.50&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[5vj6]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VJ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VJ6 FirstGlance]. <br>
@@ Line 22: / Line 22: @@
 <references/>
 __TOC__
-</StructureSection>
+</SX>
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Bjorkman, P J]]
 [[Category: Wang, H]]

5vj6

From Proteopedia

Revision as of 19:52, 6 March 2020

BG505 SOSIP.664 in complex with broadly neutralizing antibodies PG9 and 8ANC195

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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