6cbe

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{{Large structure}}
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==Atomic structure of a rationally engineered gene delivery vector, AAV2.5==
==Atomic structure of a rationally engineered gene delivery vector, AAV2.5==
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<StructureSection load='6cbe' size='340' side='right' caption='[[6cbe]], [[Resolution|resolution]] 2.78&Aring;' scene=''>
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<SX load='6cbe' size='340' side='right' viewer='molstar' caption='[[6cbe]], [[Resolution|resolution]] 2.78&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6cbe]] is a 60 chain structure with sequence from [http://en.wikipedia.org/wiki/Aav-2 Aav-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CBE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CBE FirstGlance]. <br>
<table><tr><td colspan='2'>[[6cbe]] is a 60 chain structure with sequence from [http://en.wikipedia.org/wiki/Aav-2 Aav-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CBE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CBE FirstGlance]. <br>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cbe OCA], [http://pdbe.org/6cbe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cbe RCSB], [http://www.ebi.ac.uk/pdbsum/6cbe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cbe ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cbe OCA], [http://pdbe.org/6cbe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cbe RCSB], [http://www.ebi.ac.uk/pdbsum/6cbe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cbe ProSAT]</span></td></tr>
</table>
</table>
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{{Large structure}}
 
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/CAPSD_AAV2S CAPSD_AAV2S]] Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus.<ref>PMID:10684294</ref> <ref>PMID:11961250</ref> <ref>PMID:16940508</ref> <ref>PMID:9445046</ref>
[[http://www.uniprot.org/uniprot/CAPSD_AAV2S CAPSD_AAV2S]] Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus.<ref>PMID:10684294</ref> <ref>PMID:11961250</ref> <ref>PMID:16940508</ref> <ref>PMID:9445046</ref>
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<references/>
<references/>
__TOC__
__TOC__
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</StructureSection>
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</SX>
[[Category: Aav-2]]
[[Category: Aav-2]]
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[[Category: Large Structures]]
[[Category: Agbandje-McKenna, M]]
[[Category: Agbandje-McKenna, M]]
[[Category: Bennett, A]]
[[Category: Bennett, A]]

Revision as of 20:39, 6 March 2020

Atomic structure of a rationally engineered gene delivery vector, AAV2.5

6cbe, resolution 2.78Å

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