<table><tr><td colspan='2'>[[6j6i]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J6I FirstGlance]. <br>
<table><tr><td colspan='2'>[[6j6i]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J6I FirstGlance]. <br>
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[[Category: Arath]]
[[Category: Arath]]
[[Category: Large Structures]]
[[Category: Large Structures]]
Revision as of 22:14, 6 March 2020
Reconstitution and structure of a plant NLR resistosome conferring immunity
6j6i is a 7 chain structure with sequence from Arath. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
[PBL2_ARATH] Involved in disease resistance signaling (PubMed:20413097, PubMed:23951354). Contributes to pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) signaling and defense responses downstream of FLS2 (PubMed:20413097). Required for the bacterial XopAC/AvrAC effector-triggered immunity (ETI) against Xanthomonas campestris (PubMed:23951354).[1][2] [R13L4_ARATH] CC-NB-LRR receptor-like protein required for recognition of the Pseudomonas syringae type III effector HopZ1a. Confers allele-specific recognition and virulence attenuation of HopZ1a. Immunity mediated by ZAR1 is independent of several genes required by other resistance protein signaling pathways such as NDR1 and RAR1.[3]
Publication Abstract from PubMed
Nucleotide-binding, leucine-rich repeat receptors (NLRs) perceive pathogen effectors to trigger plant immunity. Biochemical mechanisms underlying plant NLR activation have until now remained poorly understood. We reconstituted an active complex containing the Arabidopsis coiled-coil NLR ZAR1, the pseudokinase RKS1, uridylated protein kinase PBL2, and 2'-deoxyadenosine 5'-triphosphate (dATP), demonstrating the oligomerization of the complex during immune activation. The cryo-electron microscopy structure reveals a wheel-like pentameric ZAR1 resistosome. Besides the nucleotide-binding domain, the coiled-coil domain of ZAR1 also contributes to resistosome pentamerization by forming an alpha-helical barrel that interacts with the leucine-rich repeat and winged-helix domains. Structural remodeling and fold switching during activation release the very N-terminal amphipathic alpha helix of ZAR1 to form a funnel-shaped structure that is required for the plasma membrane association, cell death triggering, and disease resistance, offering clues to the biochemical function of a plant resistosome.
Reconstitution and structure of a plant NLR resistosome conferring immunity.,Wang J, Hu M, Wang J, Qi J, Han Z, Wang G, Qi Y, Wang HW, Zhou JM, Chai J Science. 2019 Apr 5;364(6435). pii: 364/6435/eaav5870. doi:, 10.1126/science.aav5870. PMID:30948527[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Zhang J, Li W, Xiang T, Liu Z, Laluk K, Ding X, Zou Y, Gao M, Zhang X, Chen S, Mengiste T, Zhang Y, Zhou JM. Receptor-like cytoplasmic kinases integrate signaling from multiple plant immune receptors and are targeted by a Pseudomonas syringae effector. Cell Host Microbe. 2010 Apr 22;7(4):290-301. doi: 10.1016/j.chom.2010.03.007. PMID:20413097 doi:http://dx.doi.org/10.1016/j.chom.2010.03.007
↑ Guy E, Lautier M, Chabannes M, Roux B, Lauber E, Arlat M, Noel LD. xopAC-triggered immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK. PLoS One. 2013 Aug 9;8(8):e73469. doi: 10.1371/journal.pone.0073469. eCollection , 2013. PMID:23951354 doi:http://dx.doi.org/10.1371/journal.pone.0073469
↑ Lewis JD, Wu R, Guttman DS, Desveaux D. Allele-specific virulence attenuation of the Pseudomonas syringae HopZ1a type III effector via the Arabidopsis ZAR1 resistance protein. PLoS Genet. 2010 Apr 1;6(4):e1000894. doi: 10.1371/journal.pgen.1000894. PMID:20368970 doi:http://dx.doi.org/10.1371/journal.pgen.1000894
↑ Wang J, Hu M, Wang J, Qi J, Han Z, Wang G, Qi Y, Wang HW, Zhou JM, Chai J. Reconstitution and structure of a plant NLR resistosome conferring immunity. Science. 2019 Apr 5;364(6435). pii: 364/6435/eaav5870. doi:, 10.1126/science.aav5870. PMID:30948527 doi:http://dx.doi.org/10.1126/science.aav5870
