6mdo

From Proteopedia

(Difference between revisions)

Revision as of 22:32, 6 March 2020

The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 1)

6mdo, resolution 3.90Å

Structural highlights

6mdo is a 7 chain structure with sequence from Buffalo rat and Cho cell lines. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3j93, 3j94, 3j96, 3j97, 3j98, 3j99
Gene:	NSF (CHO cell lines), Snap25, Snap (Buffalo rat)
Activity:	Vesicle-fusing ATPase, with EC number 3.6.4.6
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NSF_CRIGR] Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling. [SNP25_RAT] t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.

Publication Abstract from PubMed

The recycling of SNARE proteins following complex formation and membrane fusion is an essential process in eukaryotic trafficking. A highly conserved AAA+ protein, NSF (N-ethylmaleimide sensitive factor) and an adaptor protein, SNAP (soluble NSF attachment protein), disassembles the SNARE complex. We report electron-cryomicroscopy structures of the complex of NSF, alphaSNAP, and the full-length soluble neuronal SNARE complex (composed of syntaxin-1A, synaptobrevin-2, SNAP-25A) in the presence of ATP under non-hydrolyzing conditions at ~3.9 A resolution. These structures reveal electrostatic interactions by which two alphaSNAP molecules interface with a specific surface of the SNARE complex. This interaction positions the SNAREs such that the 15 N-terminal residues of SNAP-25A are loaded into the D1 ring pore of NSF via a spiral pattern of interactions between a conserved tyrosine NSF residue and SNAP-25A backbone atoms. This loading process likely precedes ATP hydrolysis. Subsequent ATP hydrolysis then drives complete disassembly.

Structural principles of SNARE complex recognition by the AAA+ protein NSF.,White KI, Zhao M, Choi UB, Pfuetzner RA, Brunger AT Elife. 2018 Sep 10;7. pii: 38888. doi: 10.7554/eLife.38888. PMID:30198481^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ White KI, Zhao M, Choi UB, Pfuetzner RA, Brunger AT. Structural principles of SNARE complex recognition by the AAA+ protein NSF. Elife. 2018 Sep 10;7. pii: 38888. doi: 10.7554/eLife.38888. PMID:30198481 doi:http://dx.doi.org/10.7554/eLife.38888

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6mdo"

@@ Line 1: / Line 1: @@
 ==The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 1)==
-<StructureSection load='6mdo' size='340' side='right'caption='[[6mdo]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
+<SX load='6mdo' size='340' side='right' viewer='molstar' caption='[[6mdo]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[6mdo]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MDO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MDO FirstGlance]. <br>
@@ Line 28: / Line 28: @@
 <references/>
 __TOC__
-</StructureSection>
+</SX>
 [[Category: Buffalo rat]]
 [[Category: Cho cell lines]]

6mdo

From Proteopedia

Revision as of 22:32, 6 March 2020

The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 1)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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