6nef

From Proteopedia

(Difference between revisions)

Revision as of 22:50, 6 March 2020

Outer Membrane Cytochrome S Filament from Geobacter Sulfurreducens

6nef, resolution 3.42Å

Structural highlights

6nef is a 1 chain structure with sequence from Geosl. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[OMCS_GEOSL] Plays an important role in extracellular electron transfer. Can transfer electrons to insoluble Fe(3+) oxides as well as other extracellular electron acceptors, including Mn(4+) oxide and humic substances (PubMed:16332857, PubMed:20400557, PubMed:21236241). Essential for direct interspecies electron transfer (DIET) in cocultures with G. metallireducens (PubMed:23377933).^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

Electrically conductive pili from Geobacter species, termed bacterial nanowires, are intensely studied for their biological significance and potential in the development of new materials. Using cryo-electron microscopy, we have characterized nanowires from conductive G. sulfurreducens pili preparations that are composed solely of head-to-tail stacked monomers of the six-heme C-type cytochrome OmcS. The unique fold of OmcS - closely wrapped around a continuous stack of hemes that can serve as an uninterrupted path for electron transport - generates a scaffold that supports the unbranched chain of hemes along the central axis of the filament. We present here, at 3.4 A resolution, the structure of this cytochrome-based filament and discuss its possible role in long-range biological electron transport.

Cryo-EM reveals the structural basis of long-range electron transport in a cytochrome-based bacterial nanowire.,Filman DJ, Marino SF, Ward JE, Yang L, Mester Z, Bullitt E, Lovley DR, Strauss M Commun Biol. 2019 Jun 19;2:219. doi: 10.1038/s42003-019-0448-9. eCollection 2019. PMID:31240257^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mehta T, Coppi MV, Childers SE, Lovley DR. Outer membrane c-type cytochromes required for Fe(III) and Mn(IV) oxide reduction in Geobacter sulfurreducens. Appl Environ Microbiol. 2005 Dec;71(12):8634-41. doi:, 10.1128/AEM.71.12.8634-8641.2005. PMID:16332857 doi:http://dx.doi.org/10.1128/AEM.71.12.8634-8641.2005
↑ Leang C, Qian X, Mester T, Lovley DR. Alignment of the c-type cytochrome OmcS along pili of Geobacter sulfurreducens. Appl Environ Microbiol. 2010 Jun;76(12):4080-4. doi: 10.1128/AEM.00023-10. Epub, 2010 Apr 16. PMID:20400557 doi:http://dx.doi.org/10.1128/AEM.00023-10
↑ Qian X, Mester T, Morgado L, Arakawa T, Sharma ML, Inoue K, Joseph C, Salgueiro CA, Maroney MJ, Lovley DR. Biochemical characterization of purified OmcS, a c-type cytochrome required for insoluble Fe(III) reduction in Geobacter sulfurreducens. Biochim Biophys Acta. 2011 Apr;1807(4):404-12. doi: 10.1016/j.bbabio.2011.01.003., Epub 2011 Jan 12. PMID:21236241 doi:http://dx.doi.org/10.1016/j.bbabio.2011.01.003
↑ Shrestha PM, Rotaru AE, Summers ZM, Shrestha M, Liu F, Lovley DR. Transcriptomic and genetic analysis of direct interspecies electron transfer. Appl Environ Microbiol. 2013 Apr;79(7):2397-404. doi: 10.1128/AEM.03837-12. Epub , 2013 Feb 1. PMID:23377933 doi:http://dx.doi.org/10.1128/AEM.03837-12
↑ Filman DJ, Marino SF, Ward JE, Yang L, Mester Z, Bullitt E, Lovley DR, Strauss M. Cryo-EM reveals the structural basis of long-range electron transport in a cytochrome-based bacterial nanowire. Commun Biol. 2019 Jun 19;2:219. doi: 10.1038/s42003-019-0448-9. eCollection 2019. PMID:31240257 doi:http://dx.doi.org/10.1038/s42003-019-0448-9

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6nef"

@@ Line 1: / Line 1: @@
 ==Outer Membrane Cytochrome S Filament from Geobacter Sulfurreducens==
-<StructureSection load='6nef' size='340' side='right'caption='[[6nef]], [[Resolution|resolution]] 3.42&Aring;' scene=''>
+<SX load='6nef' size='340' side='right' viewer='molstar' caption='[[6nef]], [[Resolution|resolution]] 3.42&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6nef]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NEF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6nef]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geosl Geosl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NEF FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nef OCA], [http://pdbe.org/6nef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nef RCSB], [http://www.ebi.ac.uk/pdbsum/6nef PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nef ProSAT]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/OMCS_GEOSL OMCS_GEOSL]] Plays an important role in extracellular electron transfer. Can transfer electrons to insoluble Fe(3+) oxides as well as other extracellular electron acceptors, including Mn(4+) oxide and humic substances (PubMed:16332857, PubMed:20400557, PubMed:21236241). Essential for direct interspecies electron transfer (DIET) in cocultures with G. metallireducens (PubMed:23377933).<ref>PMID:16332857</ref> <ref>PMID:20400557</ref> <ref>PMID:21236241</ref> <ref>PMID:23377933</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 16: / Line 18: @@
 </div>
 <div class="pdbe-citations 6nef" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Highest impact structures|Highest impact structures]]
+*[[User:Nikhil Malvankar/Cytochrome nanowires|User:Nikhil Malvankar/Cytochrome nanowires]]
 == References ==
 <references/>
 __TOC__
-</StructureSection>
+</SX>
-[[Category: Geobacter sulfurreducens]]
+[[Category: Geosl]]
 [[Category: Large Structures]]
 [[Category: Bullitt, E]]

6nef

From Proteopedia

Revision as of 22:50, 6 March 2020

Outer Membrane Cytochrome S Filament from Geobacter Sulfurreducens

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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