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6v6s

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Revision as of 01:18, 7 March 2020

Structure of the native human gamma-tubulin ring complex

6v6s, resolution 4.30Å

Structural highlights

6v6s is a 38 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[GCP6_HUMAN] Autosomal recessive chorioretinopathy-microcephaly syndrome. The disease is caused by mutations affecting the gene represented in this entry.

Function

[TBG1_HUMAN] Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation. [GCP4_HUMAN] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. [GCP3_HUMAN] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. [GCP2_HUMAN] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. [GCP5_HUMAN] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. [GCP6_HUMAN] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.^[1]

Publication Abstract from PubMed

The gamma-tubulin ring complex (gamma-TuRC) is an essential regulator of centrosomal and acentrosomal microtubule formation, yet its structure is not known. Here, we present a cryo-EM reconstruction of the native human gamma-TuRC at approximately 3.8 A resolution, revealing an asymmetric, cone-shaped structure. Pseudo-atomic models indicate that GCP4, GCP5, and GCP6 form distinct Y-shaped assemblies that structurally mimic GCP2/GCP3 subcomplexes distal to the gamma-TuRC "seam." We also identify an unanticipated structural bridge that includes an actin-like protein and spans the gamma-TuRC lumen. Despite its asymmetric architecture, the gamma-TuRC arranges gamma-tubulins into a helical geometry poised to nucleate microtubules. Diversity in the gamma-TuRC subunits introduces large (>100,000 A(2)) surfaces in the complex that allow for interactions with different regulatory factors. The observed compositional complexity of the gamma-TuRC could self-regulate its assembly into a cone-shaped structure to control microtubule formation across diverse contexts, e.g., within biological condensates or alongside existing filaments.

Asymmetric Molecular Architecture of the Human gamma-Tubulin Ring Complex.,Wieczorek M, Urnavicius L, Ti SC, Molloy KR, Chait BT, Kapoor TM Cell. 2019 Dec 13. pii: S0092-8674(19)31369-8. doi: 10.1016/j.cell.2019.12.007. PMID:31862189^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Murphy SM, Preble AM, Patel UK, O'Connell KL, Dias DP, Moritz M, Agard D, Stults JT, Stearns T. GCP5 and GCP6: two new members of the human gamma-tubulin complex. Mol Biol Cell. 2001 Nov;12(11):3340-52. PMID:11694571
↑ Wieczorek M, Urnavicius L, Ti SC, Molloy KR, Chait BT, Kapoor TM. Asymmetric Molecular Architecture of the Human gamma-Tubulin Ring Complex. Cell. 2019 Dec 13. pii: S0092-8674(19)31369-8. doi: 10.1016/j.cell.2019.12.007. PMID:31862189 doi:http://dx.doi.org/10.1016/j.cell.2019.12.007

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6v6s"

@@ Line 1: / Line 1: @@
 ==Structure of the native human gamma-tubulin ring complex==
-<StructureSection load='6v6s' size='340' side='right'caption='[[6v6s]], [[Resolution|resolution]] 4.30&Aring;' scene=''>
+<SX load='6v6s' size='340' side='right' viewer='molstar' caption='[[6v6s]], [[Resolution|resolution]] 4.30&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[6v6s]] is a 38 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V6S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6V6S FirstGlance]. <br>
@@ Line 24: / Line 24: @@
 <references/>
 __TOC__
-</StructureSection>
+</SX>
 [[Category: Homo sapiens]]
 [[Category: Large Structures]]

6v6s

From Proteopedia

Revision as of 01:18, 7 March 2020

Structure of the native human gamma-tubulin ring complex

Structural highlights

Disease

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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