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2ejy

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Revision as of 08:34, 11 March 2020

Solution structure of the p55 PDZ T85C domain complexed with the glycophorin C F127C peptide

Structural highlights

2ejy is a 2 chain structure with sequence from Human. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2ev8
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[EM55_HUMAN] Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity (By similarity). [GLPC_HUMAN] This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

p55, a member of the membrane-associated guanylate kinase family, includes a PDZ domain that specifically interacts with the C-terminal region of glycophorin C in the ternary complex of p55, protein 4.1 and glycophorin C. Here we present the first NMR-derived complex structure of the p55 PDZ domain and the C-terminal peptide of glycophorin C, obtained by using a threonine to cysteine (T85C) mutant of the p55 PDZ domain and a phenylalanine to cysteine (F127C) mutant of the glycophorin C peptide. Our NMR results revealed that the two designed mutant molecules retain the specific interaction manner that exists between the wild type molecules and can facilitate the structure determination by NMR, due to the stable complex formation via an intermolecular disulfide bond. The complex structure provides insight into the specific interaction of the p55 PDZ domain with the two key residues, Ile128 and Tyr126, of glycophorin C.

Structural insight into the interaction between the p55 PDZ domain and glycophorin C.,Kusunoki H, Kohno T Biochem Biophys Res Commun. 2007 Aug 10;359(4):972-8. Epub 2007 Jun 11. PMID:17572384^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kusunoki H, Kohno T. Structural insight into the interaction between the p55 PDZ domain and glycophorin C. Biochem Biophys Res Commun. 2007 Aug 10;359(4):972-8. Epub 2007 Jun 11. PMID:17572384 doi:10.1016/j.bbrc.2007.05.215

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ejy"

@@ Line 1: / Line 1: @@
 ==Solution structure of the p55 PDZ T85C domain complexed with the glycophorin C F127C peptide==
-<StructureSection load='2ejy' size='340' side='right' caption='[[2ejy]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2ejy' size='340' side='right'caption='[[2ejy]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2ejy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EJY FirstGlance]. <br>
@@ Line 33: / Line 33: @@
 </StructureSection>
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Kohno, T]]
 [[Category: Kusunoki, H]]

2ejy

From Proteopedia

Revision as of 08:34, 11 March 2020

Solution structure of the p55 PDZ T85C domain complexed with the glycophorin C F127C peptide

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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