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5aim

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Revision as of 08:40, 11 March 2020

Crystal structure of T138 central eWH domain

Structural highlights

5aim is a 2 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	5aio
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TFC3_YEAST] TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauB domain of TFIIIC that binds boxB DNA promoter sites of tRNA and similar genes. TFC3 is essential for cell viability. Cooperates with TFC6 in DNA binding.^[1] ^[2] ^[3]

Publication Abstract from PubMed

In eukaryotes, RNA Polymerase III (Pol III) is specifically responsible for transcribing genes encoding tRNAs and other short non-coding RNAs. The recruitment of Pol III to tRNA-encoding genes requires the transcription factors (TF) IIIB and IIIC. TFIIIC has been described as a conserved, multi-subunit protein complex composed of two subcomplexes, called tauA and tauB. How these two subcomplexes are linked and how their interaction affects the formation of the Pol III pre-initiation complex (PIC) is poorly understood. Here we use chemical crosslinking mass spectrometry and determine the molecular architecture of TFIIIC. We further report the crystal structure of the essential TPR array from tauA subunit tau131 and characterize its interaction with a central region of tauB subunit tau138. The identified tau131-tau138 interacting region is essential in vivo and overlaps with TFIIIB-binding sites, revealing a crucial interaction platform for the regulation of tRNA transcription initiation.

Architecture of TFIIIC and its role in RNA polymerase III pre-initiation complex assembly.,Male G, von Appen A, Glatt S, Taylor NM, Cristovao M, Groetsch H, Beck M, Muller CW Nat Commun. 2015 Jun 10;6:7387. doi: 10.1038/ncomms8387. PMID:26060179^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lefebvre O, Carles C, Conesa C, Swanson RN, Bouet F, Riva M, Sentenac A. TFC3: gene encoding the B-block binding subunit of the yeast transcription factor IIIC. Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10512-6. PMID:1279682
↑ Gabrielsen OS, Marzouki N, Ruet A, Sentenac A, Fromageot P. Two polypeptide chains in yeast transcription factor tau interact with DNA. J Biol Chem. 1989 May 5;264(13):7505-11. PMID:2651441
↑ Arrebola R, Manaud N, Rozenfeld S, Marsolier MC, Lefebvre O, Carles C, Thuriaux P, Conesa C, Sentenac A. Tau91, an essential subunit of yeast transcription factor IIIC, cooperates with tau138 in DNA binding. Mol Cell Biol. 1998 Jan;18(1):1-9. PMID:9418847
↑ Male G, von Appen A, Glatt S, Taylor NM, Cristovao M, Groetsch H, Beck M, Muller CW. Architecture of TFIIIC and its role in RNA polymerase III pre-initiation complex assembly. Nat Commun. 2015 Jun 10;6:7387. doi: 10.1038/ncomms8387. PMID:26060179 doi:http://dx.doi.org/10.1038/ncomms8387

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5aim"

@@ Line 1: / Line 1: @@
 ==Crystal structure of T138 central eWH domain==
-<StructureSection load='5aim' size='340' side='right' caption='[[5aim]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
+<StructureSection load='5aim' size='340' side='right'caption='[[5aim]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[5aim]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AIM FirstGlance]. <br>
@@ Line 19: / Line 19: @@
 </div>
 <div class="pdbe-citations 5aim" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Transcription factor tau|Transcription factor tau]]
 == References ==
 <references/>
@@ Line 24: / Line 27: @@
 </StructureSection>
 [[Category: Atcc 18824]]
+[[Category: Large Structures]]
 [[Category: Glatt, S]]
 [[Category: Male, G]]

5aim

From Proteopedia

Revision as of 08:40, 11 March 2020

Crystal structure of T138 central eWH domain

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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