6qrm
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==HsNMT1 in complex with both MyrCoA and GNCFSKRRAA substrates== | |
| + | <StructureSection load='6qrm' size='340' side='right'caption='[[6qrm]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6qrm]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QRM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QRM FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MYA:TETRADECANOYL-COA'>MYA</scene>, <scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycylpeptide_N-tetradecanoyltransferase Glycylpeptide N-tetradecanoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.97 2.3.1.97] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qrm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qrm OCA], [http://pdbe.org/6qrm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qrm RCSB], [http://www.ebi.ac.uk/pdbsum/6qrm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qrm ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/NMT1_HUMAN NMT1_HUMAN]] Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins. [[http://www.uniprot.org/uniprot/AIFM3_HUMAN AIFM3_HUMAN]] Induces apoptosis through a caspase dependent pathway. Reduces mitochondrial membrane potential.<ref>PMID:15764604</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The promising drug target N-myristoyltransferase (NMT) catalyses an essential protein modification thought to occur exclusively at N-terminal glycines (Gly). Here, we present high-resolution human NMT1 structures co-crystallised with reactive cognate lipid and peptide substrates, revealing high-resolution snapshots of the entire catalytic mechanism from the initial to final reaction states. Structural comparisons, together with biochemical analysis, provide unforeseen details about how NMT1 reaches a catalytically competent conformation in which the reactive groups are brought into close proximity to enable catalysis. We demonstrate that this mechanism further supports efficient and unprecedented myristoylation of an N-terminal lysine side chain, providing evidence that NMT acts both as N-terminal-lysine and glycine myristoyltransferase. | ||
| - | + | High-resolution snapshots of human N-myristoyltransferase in action illuminate a mechanism promoting N-terminal Lys and Gly myristoylation.,Dian C, Perez-Dorado I, Riviere F, Asensio T, Legrand P, Ritzefeld M, Shen M, Cota E, Meinnel T, Tate EW, Giglione C Nat Commun. 2020 Feb 28;11(1):1132. doi: 10.1038/s41467-020-14847-3. PMID:32111831<ref>PMID:32111831</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6qrm" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Glycylpeptide N-tetradecanoyltransferase]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Asensio, T]] | [[Category: Asensio, T]] | ||
| - | [[Category: Meinnel, T]] | ||
[[Category: Dian, C]] | [[Category: Dian, C]] | ||
[[Category: Giglione, C]] | [[Category: Giglione, C]] | ||
| - | [[Category: Riviere, F | + | [[Category: Meinnel, T]] |
| + | [[Category: Riviere, F B]] | ||
| + | [[Category: Acyltransferase]] | ||
| + | [[Category: Gcn5-related n-acetyltransferase]] | ||
| + | [[Category: Gnat]] | ||
| + | [[Category: Myristoyltransferase type1]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 09:46, 18 March 2020
HsNMT1 in complex with both MyrCoA and GNCFSKRRAA substrates
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