6u1r
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==SxtG an amidinotransferase from the Microseira wollei in Saxitoxin biosynthetic pathway== | |
| + | <StructureSection load='6u1r' size='340' side='right'caption='[[6u1r]], [[Resolution|resolution]] 1.79Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6u1r]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U1R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6U1R FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6u1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u1r OCA], [http://pdbe.org/6u1r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u1r RCSB], [http://www.ebi.ac.uk/pdbsum/6u1r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u1r ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Secondary metabolites are assembled by enzymes that often perform reactions with high selectivity and specificity. Many of these enzymes also tolerate variations in substrate structure, exhibiting promiscuity that enables various applications of a given biocatalyst. However, initial enzyme characterization studies frequently do not explore beyond the native substrates. This limited assessment of substrate scope contributes to the difficulty of identifying appropriate enzymes for specific synthetic applications. Here, we report the natural function of cyanobacterial SxtG, an amidinotransferase involved in the biosynthesis of paralytic shellfish toxins, and demonstrate its ability to modify a breadth of non-native substrates. In addition, we report the first X-ray crystal structure of SxtG, which provides rationale for this enzyme's substrate scope. Taken together, these data confirm the function of SxtG and exemplify its potential utility in biocatalytic synthesis. | ||
| - | + | Substrate Promiscuity of a Paralytic Shellfish Toxin Amidinotransferase.,Lukowski AL, Mallik L, Hinze ME, Carlson BM, Ellinwood DC, Pyser JB, Koutmos M, Narayan ARH ACS Chem Biol. 2020 Feb 14. doi: 10.1021/acschembio.9b00964. PMID:32058687<ref>PMID:32058687</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6u1r" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Koutmos, M]] | ||
| + | [[Category: Lukowski, A L]] | ||
| + | [[Category: Mallik, L]] | ||
| + | [[Category: Narayan, A R.H]] | ||
| + | [[Category: Amidinotransferase]] | ||
| + | [[Category: Saxitoxin biosynthetic pathway]] | ||
| + | [[Category: Sxtg]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 09:56, 18 March 2020
SxtG an amidinotransferase from the Microseira wollei in Saxitoxin biosynthetic pathway
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