5but

From Proteopedia

(Difference between revisions)

Revision as of 11:01, 18 March 2020

Crystal structure of inactive conformation of KtrAB K+ transporter

Structural highlights

5but is a 8 chain structure with sequence from "vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	ktrA, yuaA, BSU31090 ("Vibrio subtilis" Ehrenberg 1835), ktrB, yubG, BSU31100 ("Vibrio subtilis" Ehrenberg 1835)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[KTRA_BACSU] Catalytic subunit of the KtrAB potassium uptake transporter. The 2 major potassium transporter complexes KtrAB and KtrCD confer resistance to both suddenly imposed and prolonged osmotic stress.^[1] [KTRB_BACSU] Integral membrane subunit of the KtrAB potassium uptake transporter. The 2 major potassium transporter complexes KtrAB and KtrCD confer resistance to both suddenly imposed and prolonged osmotic stress.^[2] ^[3]

Publication Abstract from PubMed

KtrAB belongs to the Trk/Ktr/HKT superfamily of monovalent cation (K+ and Na+) transport proteins that closely resemble K+ channels. These proteins underlie a plethora of cellular functions that are crucial for environmental adaptation in plants, fungi, archaea, and bacteria. The activation mechanism of the Trk/Ktr/HKT proteins remains unknown. It has been shown that ATP stimulates the activity of KtrAB while ADP does not. Here, we present X-ray structural information on the KtrAB complex with bound ADP. A comparison with the KtrAB-ATP structure reveals conformational changes in the ring and in the membrane protein. In combination with a biochemical and functional analysis, we uncover how ligand-dependent changes in the KtrA ring are propagated to the KtrB membrane protein and conclude that, despite their structural similarity, the activation mechanism of KtrAB is markedly different from the activation mechanism of K+ channels.

Dissecting the Molecular Mechanism of Nucleotide-Dependent Activation of the KtrAB K+ Transporter.,Szollosi A, Vieira-Pires RS, Teixeira-Duarte CM, Rocha R, Morais-Cabral JH PLoS Biol. 2016 Jan 15;14(1):e1002356. doi: 10.1371/journal.pbio.1002356., eCollection 2016 Jan. PMID:26771197^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Holtmann G, Bakker EP, Uozumi N, Bremer E. KtrAB and KtrCD: two K+ uptake systems in Bacillus subtilis and their role in adaptation to hypertonicity. J Bacteriol. 2003 Feb;185(4):1289-98. PMID:12562800
↑ Holtmann G, Bakker EP, Uozumi N, Bremer E. KtrAB and KtrCD: two K+ uptake systems in Bacillus subtilis and their role in adaptation to hypertonicity. J Bacteriol. 2003 Feb;185(4):1289-98. PMID:12562800
↑ Albright RA, Joh K, Morais-Cabral JH. Probing the structure of the dimeric KtrB membrane protein. J Biol Chem. 2007 Nov 30;282(48):35046-55. Epub 2007 Oct 10. PMID:17932047 doi:10.1074/jbc.M704260200
↑ Szollosi A, Vieira-Pires RS, Teixeira-Duarte CM, Rocha R, Morais-Cabral JH. Dissecting the Molecular Mechanism of Nucleotide-Dependent Activation of the KtrAB K+ Transporter. PLoS Biol. 2016 Jan 15;14(1):e1002356. doi: 10.1371/journal.pbio.1002356., eCollection 2016 Jan. PMID:26771197 doi:http://dx.doi.org/10.1371/journal.pbio.1002356

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5but"

@@ Line 1: / Line 1: @@
 ==Crystal structure of inactive conformation of KtrAB K+ transporter==
-<StructureSection load='5but' size='340' side='right' caption='[[5but]], [[Resolution|resolution]] 5.97&Aring;' scene=''>
+<StructureSection load='5but' size='340' side='right'caption='[[5but]], [[Resolution|resolution]] 5.97&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5but]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BUT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BUT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5but]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BUT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BUT FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5but FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5but OCA], [http://pdbe.org/5but PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5but RCSB], [http://www.ebi.ac.uk/pdbsum/5but PDBsum]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ktrA, yuaA, BSU31090 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835]), ktrB, yubG, BSU31100 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5but FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5but OCA], [http://pdbe.org/5but PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5but RCSB], [http://www.ebi.ac.uk/pdbsum/5but PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5but ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Vibrio subtilis ehrenberg 1835]]
+[[Category: Large Structures]]
 [[Category: Morais-Cabral, J H]]
 [[Category: Vieira-Pires, R S]]
 [[Category: Membrane protein]]
 [[Category: Membrane protein complex]]

5but

From Proteopedia

Revision as of 11:01, 18 March 2020

Crystal structure of inactive conformation of KtrAB K+ transporter

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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