This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

5c6g

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 11:13, 18 March 2020

Structural Insights into the Scc2-Scc4 Cohesin Loader

Structural highlights

5c6g is a 4 chain structure with sequence from Ashgo. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	AGOS_AGR133C (ASHGO), SCC2, AGL133W (ASHGO)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SCC2_ASHGO] Plays a structural role in chromatin. Probably involved in sister chromatid cohesion, possibly by interacting with the cohesin complex (By similarity).

Publication Abstract from PubMed

The remarkable accuracy of eukaryotic cell division is partly maintained by the cohesin complex acting as a molecular glue to prevent premature sister chromatid separation. The loading of cohesin onto chromosomes is catalyzed by the Scc2-Scc4 loader complex. Here, we report the crystal structure of Scc4 bound to the N terminus of Scc2 and show that Scc4 is a tetratricopeptide repeat (TPR) superhelix. The Scc2 N terminus adopts an extended conformation and is entrapped by the core of the Scc4 superhelix. Electron microscopy (EM) analysis reveals that the Scc2-Scc4 loader complex comprises three domains: a head, body, and hook. Deletion studies unambiguously assign the Scc2N-Scc4 as the globular head domain, whereas in vitro cohesin loading assays show that the central body and the hook domains are sufficient to catalyze cohesin loading onto circular DNA, but not chromatinized DNA in vivo, suggesting a possible role for Scc4 as a chromatin adaptor.

Structural Studies Reveal the Functional Modularity of the Scc2-Scc4 Cohesin Loader.,Chao WC, Murayama Y, Munoz S, Costa A, Uhlmann F, Singleton MR Cell Rep. 2015 Jul 22. pii: S2211-1247(15)00709-3. doi:, 10.1016/j.celrep.2015.06.071. PMID:26212329^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chao WC, Murayama Y, Munoz S, Costa A, Uhlmann F, Singleton MR. Structural Studies Reveal the Functional Modularity of the Scc2-Scc4 Cohesin Loader. Cell Rep. 2015 Jul 22. pii: S2211-1247(15)00709-3. doi:, 10.1016/j.celrep.2015.06.071. PMID:26212329 doi:http://dx.doi.org/10.1016/j.celrep.2015.06.071

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5c6g"

@@ Line 1: / Line 1: @@
 ==Structural Insights into the Scc2-Scc4 Cohesin Loader==
-<StructureSection load='5c6g' size='340' side='right' caption='[[5c6g]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+<StructureSection load='5c6g' size='340' side='right'caption='[[5c6g]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5c6g]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C6G FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5c6g]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ashgo Ashgo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C6G FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c6g OCA], [http://pdbe.org/5c6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c6g RCSB], [http://www.ebi.ac.uk/pdbsum/5c6g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c6g ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AGOS_AGR133C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284811 ASHGO]), SCC2, AGL133W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284811 ASHGO])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c6g OCA], [http://pdbe.org/5c6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c6g RCSB], [http://www.ebi.ac.uk/pdbsum/5c6g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c6g ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Ashgo]]
+[[Category: Large Structures]]
 [[Category: Chao, W C.H]]
 [[Category: Singleton, M R]]

5c6g

From Proteopedia

Revision as of 11:13, 18 March 2020

Structural Insights into the Scc2-Scc4 Cohesin Loader

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox