1auj

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[[Image:1auj.gif|left|200px]]
[[Image:1auj.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1auj |SIZE=350|CAPTION= <scene name='initialview01'>1auj</scene>, resolution 2.1&Aring;
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The line below this paragraph, containing "STRUCTURE_1auj", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PPB:1-{[1-(2-AMINO-3-PHENYL-PROPIONYL)-PYRROLIDINE-2-CARBONYL]-AMINO}-2-(3-CYANO-PHENYL)-ETHANEBORONIC+ACID'>PPB</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1auj| PDB=1auj | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1auj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1auj OCA], [http://www.ebi.ac.uk/pdbsum/1auj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1auj RCSB]</span>
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}}
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'''BOVINE TRYPSIN COMPLEXED TO META-CYANO-BENZYLIC INHIBITOR'''
'''BOVINE TRYPSIN COMPLEXED TO META-CYANO-BENZYLIC INHIBITOR'''
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[[Category: Kettner, C.]]
[[Category: Kettner, C.]]
[[Category: Smallwood, A.]]
[[Category: Smallwood, A.]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: serine proteinase]]
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[[Category: Serine proteinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:42:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:47:45 2008''
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Revision as of 07:42, 2 May 2008

Image:1auj.gif

Template:STRUCTURE 1auj

BOVINE TRYPSIN COMPLEXED TO META-CYANO-BENZYLIC INHIBITOR


Overview

Highly effective thrombin inhibitors have been obtained by preparing boronic acid analogues of m-cyano-substituted phenylalanine and its incorporation into peptides. The cyano group enhances binding by several orders of magnitude. For example, Ac-(D)Phe-Pro-boroPheOH binds to thrombin with a Ki of 320 nM and the Ki of Ac-(D)Phe-Pro-boroPhe(m-CN)-OH is 0.79 nM. Protein crystal structure determination of trypsin complexed to H-(D)Phe-Pro-boroPhe(m-CN)-OH indicates that the aromatic side chain is bound in the P1 binding site and that the cyano group can act as a H-bond acceptor for the amide proton of Gly219. Enhanced binding for inhibitors containing the m-cyano group was observed for coagulation factor Xa and for the factor VIIa.tissue factor complex [Ki values of Ac-(D)Phe-Pro-boroPhe(mCN)-OH are 760 and 3.3 nM, respectively]. This result is consistent with the sequence homology of these two enzymes in the P1 binding site. Two enzymes lacking the strict homology in the P1 binding site, pancreatic kallikrein and chymotrypsin, did not exhibit significantly enhanced binding.

About this Structure

1AUJ is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

New inhibitors of thrombin and other trypsin-like proteases: hydrogen bonding of an aromatic cyano group with a backbone amide of the P1 binding site replaces binding of a basic side chain., Lee SL, Alexander RS, Smallwood A, Trievel R, Mersinger L, Weber PC, Kettner C, Biochemistry. 1997 Oct 28;36(43):13180-6. PMID:9341205 Page seeded by OCA on Fri May 2 10:42:22 2008

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