6l2f
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of a cupin protein (tm1459, H54AH58A mutant) in copper (Cu) substituted form== | |
| + | <StructureSection load='6l2f' size='340' side='right'caption='[[6l2f]], [[Resolution|resolution]] 1.23Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6l2f]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L2F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L2F FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l2f OCA], [http://pdbe.org/6l2f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l2f RCSB], [http://www.ebi.ac.uk/pdbsum/6l2f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l2f ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cupin superfamily proteins (TM1459) work as a macromolecular ligand framework with a double-stranded beta-barrel structure ligating to a Cu ion through histidine side chains. Variegating the first coordination sphere of TM1459 revealed that H52A and H54A/H58A mutants effectively catalyzed the diastereo- and enantioselective Michael addition reaction of nitroalkanes to an alpha,beta-unsaturated ketone. Moreover, calculated substrate docking signified C106N and F104W single-point mutations, which inverted the diastereoselectivity of H52A and further improved the stereoselectivity of H54A/H58A, respectively. | ||
| - | + | Cupin Variants as a Macromolecular Ligand Library for Stereoselective Michael Addition of Nitroalkanes.,Fujieda N, Ichihashi H, Yuasa M, Nishikawa Y, Kurisu G, Itoh S Angew Chem Int Ed Engl. 2020 Feb 19. doi: 10.1002/anie.202000129. PMID:32073197<ref>PMID:32073197</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6l2f" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Fujieda, N]] | ||
[[Category: Ichihashi, H]] | [[Category: Ichihashi, H]] | ||
[[Category: Itoh, S]] | [[Category: Itoh, S]] | ||
| - | [[Category: Nishikawa, Y]] | ||
[[Category: Kurisu, G]] | [[Category: Kurisu, G]] | ||
| - | [[Category: | + | [[Category: Nishikawa, Y]] |
| + | [[Category: Artificial metalloenzyme]] | ||
| + | [[Category: Copper enzyme]] | ||
| + | [[Category: Cupin]] | ||
| + | [[Category: Metal binding protein]] | ||
Revision as of 09:08, 1 April 2020
Crystal structure of a cupin protein (tm1459, H54AH58A mutant) in copper (Cu) substituted form
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