2wyy

From Proteopedia

(Difference between revisions)

Revision as of 17:06, 10 April 2020

CRYOEM MODEL OF THE VESICULAR STOMATITIS VIRUS

2wyy, resolution 10.00Å

Structural highlights

2wyy is a 12 chain structure with sequence from Vesicular stomatitis indiana virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1fzm, 1fzj
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NCAP_VSIVA] Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Replication is dependent on intracellular concentration of newly synthesized N, termed N(0), which corresponds to the protein not associated with RNA. In contrast, when associated with RNA, it is termed N. During replication, encapsidation by N(0) is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).

Evolutionary Conservation

Checkto colour the structure by Evolutionary Conservation, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Vesicular stomatitis virus (VSV) is a bullet-shaped rhabdovirus and a model system of negative-strand RNA viruses. Through direct visualization by means of cryo-electron microscopy, we show that each virion contains two nested, left-handed helices: an outer helix of matrix protein M and an inner helix of nucleoprotein N and RNA. M has a hub domain with four contact sites that link to neighboring M and N subunits, providing rigidity by clamping adjacent turns of the nucleocapsid. Side-by-side interactions between neighboring N subunits are critical for the nucleocapsid to form a bullet shape, and structure-based mutagenesis results support this description. Together, our data suggest a mechanism of VSV assembly in which the nucleocapsid spirals from the tip to become the helical trunk, both subsequently framed and rigidified by the M layer.

Cryo-EM model of the bullet-shaped vesicular stomatitis virus.,Ge P, Tsao J, Schein S, Green TJ, Luo M, Zhou ZH Science. 2010 Feb 5;327(5966):689-93. PMID:20133572^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ge P, Tsao J, Schein S, Green TJ, Luo M, Zhou ZH. Cryo-EM model of the bullet-shaped vesicular stomatitis virus. Science. 2010 Feb 5;327(5966):689-93. PMID:20133572 doi:327/5966/689

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wyy"

@@ Line 3: / Line 3: @@
 <SX load='2wyy' size='340' side='right' viewer='molstar' caption='[[2wyy]], [[Resolution|resolution]] 10.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2wyy]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WYY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2wyy]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2WYY FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1fzm|1fzm]], [[1fzj|1fzj]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wyy OCA], [http://pdbe.org/2wyy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wyy RCSB], [http://www.ebi.ac.uk/pdbsum/2wyy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wyy ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2wyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wyy OCA], [http://pdbe.org/2wyy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wyy RCSB], [http://www.ebi.ac.uk/pdbsum/2wyy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wyy ProSAT]</span></td></tr>
 </table>
 == Function ==

2wyy

From Proteopedia

Revision as of 17:06, 10 April 2020

CRYOEM MODEL OF THE VESICULAR STOMATITIS VIRUS

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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