3cnf

From Proteopedia

(Difference between revisions)

Revision as of 17:15, 10 April 2020

3.88 Angstrom structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy

3cnf, resolution 3.88Å

Structural highlights

3cnf is a 3 chain structure with sequence from Bombyx mori cypovirus 1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CAPSD_CPVBM] Capsid protein self-assembles to form an icosahedral capsid with a pseudo T=2 symmetry, about 50 nm in diameter, and consisting of 120 capsid proteins. The capsid encapsulates the genomic RNA.

Evolutionary Conservation

Checkto colour the structure by Evolutionary Conservation, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Cytoplasmic polyhedrosis virus (CPV) is unique within the Reoviridae family in having a turreted single-layer capsid contained within polyhedrin inclusion bodies, yet being fully capable of cell entry and endogenous RNA transcription. Biochemical data have shown that the amino-terminal 79 residues of the CPV turret protein (TP) is sufficient to bring CPV or engineered proteins into the polyhedrin matrix for micro-encapsulation. Here we report the three-dimensional structure of CPV at 3.88 A resolution using single-particle cryo-electron microscopy. Our map clearly shows the turns and deep grooves of alpha-helices, the strand separation in beta-sheets, and densities for loops and many bulky side chains; thus permitting atomic model-building effort from cryo-electron microscopy maps. We observed a helix-to-beta-hairpin conformational change between the two conformational states of the capsid shell protein in the region directly interacting with genomic RNA. We have also discovered a messenger RNA release hole coupled with the mRNA capping machinery unique to CPV. Furthermore, we have identified the polyhedrin-binding domain, a structure that has potential in nanobiotechnology applications.

3.88 A structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy.,Yu X, Jin L, Zhou ZH Nature. 2008 May 15;453(7193):415-9. Epub 2008 Apr 30. PMID:18449192^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yu X, Jin L, Zhou ZH. 3.88 A structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy. Nature. 2008 May 15;453(7193):415-9. Epub 2008 Apr 30. PMID:18449192 doi:10.1038/nature06893

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3cnf"

@@ Line 3: / Line 3: @@
 <SX load='3cnf' size='340' side='right' viewer='molstar' caption='[[3cnf]], [[Resolution|resolution]] 3.88&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3cnf]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bombyx_mori_cypovirus_1 Bombyx mori cypovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CNF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3cnf]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bombyx_mori_cypovirus_1 Bombyx mori cypovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CNF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3CNF FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cnf OCA], [http://pdbe.org/3cnf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cnf RCSB], [http://www.ebi.ac.uk/pdbsum/3cnf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cnf ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3cnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cnf OCA], [http://pdbe.org/3cnf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cnf RCSB], [http://www.ebi.ac.uk/pdbsum/3cnf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cnf ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 29: / Line 29: @@
 ==See Also==
-*[[Virus coat protein|Virus coat protein]]
+*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
 == References ==
 <references/>

3cnf

From Proteopedia

Revision as of 17:15, 10 April 2020

3.88 Angstrom structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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