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3j97

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Revision as of 18:12, 10 April 2020

Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State II)

3j97, resolution 7.80Å

Structural highlights

3j97 is a 13 chain structure with sequence from Buffalo rat and Cho cell lines. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	3j94, 3j95, 3j96, 3j98, 3j99
Gene:	NSF (CHO cell lines), Napa, Snap, Snapa (Buffalo rat), Vamp2, Syb2 (Buffalo rat), Stx1a, Sap (Buffalo rat), Snap25, Snap (Buffalo rat)
Activity:	Vesicle-fusing ATPase, with EC number 3.6.4.6
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[STX1A_RAT] Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm. [NSF_CRIGR] Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling. [VAMP2_RAT] Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). [SNAA_RAT] Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.

Publication Abstract from PubMed

Evolutionarily conserved SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptors) proteins form a complex that drives membrane fusion in eukaryotes. The ATPase NSF (N-ethylmaleimide sensitive factor), together with SNAPs (soluble NSF attachment protein), disassembles the SNARE complex into its protein components, making individual SNAREs available for subsequent rounds of fusion. Here we report structures of ATP- and ADP-bound NSF, and the NSF/SNAP/SNARE (20S) supercomplex determined by single-particle electron cryomicroscopy at near-atomic to sub-nanometre resolution without imposing symmetry. Large, potentially force-generating, conformational differences exist between ATP- and ADP-bound NSF. The 20S supercomplex exhibits broken symmetry, transitioning from six-fold symmetry of the NSF ATPase domains to pseudo four-fold symmetry of the SNARE complex. SNAPs interact with the SNARE complex with an opposite structural twist, suggesting an unwinding mechanism. The interfaces between NSF, SNAPs, and SNAREs exhibit characteristic electrostatic patterns, suggesting how one NSF/SNAP species can act on many different SNARE complexes.

Mechanistic insights into the recycling machine of the SNARE complex.,Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT Nature. 2015 Jan 12. doi: 10.1038/nature14148. PMID:25581794^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT. Mechanistic insights into the recycling machine of the SNARE complex. Nature. 2015 Jan 12. doi: 10.1038/nature14148. PMID:25581794 doi:http://dx.doi.org/10.1038/nature14148

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j97"

@@ Line 3: / Line 3: @@
 <SX load='3j97' size='340' side='right' viewer='molstar' caption='[[3j97]], [[Resolution|resolution]] 7.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3j97]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J97 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J97 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3j97]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J97 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3J97 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j94|3j94]], [[3j95|3j95]], [[3j96|3j96]], [[3j98|3j98]], [[3j99|3j99]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NSF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10029 CHO cell lines]), Napa, Snap, Snapa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Vamp2, Syb2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Stx1a, Sap ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Snap25, Snap ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j97 OCA], [http://pdbe.org/3j97 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j97 RCSB], [http://www.ebi.ac.uk/pdbsum/3j97 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j97 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3j97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j97 OCA], [http://pdbe.org/3j97 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j97 RCSB], [http://www.ebi.ac.uk/pdbsum/3j97 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j97 ProSAT]</span></td></tr>
 </table>
 == Function ==

3j97

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Revision as of 18:12, 10 April 2020

Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State II)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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