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4a7h

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Revision as of 18:46, 10 April 2020

Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 2)

4a7h, resolution 7.80Å

Structural highlights

4a7h is a 10 chain structure with sequence from Dicdi, European rabbit and Oryctolagus cuniculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1alm, 1atn, 1eqy, 1esv, 1h1v, 1ijj, 1j6z, 1kxp, 1lcu, 1lkx, 1lot, 1m8q, 1ma9, 1mvw, 1nwk, 1o18, 1o19, 1o1a, 1o1b, 1o1c, 1o1d, 1o1e, 1o1f, 1o1g, 1p8z, 1qz5, 1qz6, 1rdw, 1rfq, 1rgi, 1s22, 1sqk, 1t44, 1uy5, 1wua, 1y64, 2a3z, 2a40, 2a41, 2a42, 2a5x, 2asm, 2aso, 2asp, 2d1k, 2d3e, 2ff3, 2ff6, 2fxu, 2tma, 2v51, 2v52, 2vcp, 2vyp, 2w49, 2w4u, 2y83, 2yje, 2yjf, 4a7l, 4a7n
Activity:	Myosin ATPase, with EC number 3.6.4.1
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ACTS_RABIT] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [MYOE_DICDI] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin. May play a role in moving membranes relative to actin. [TPM1_RABIT] Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

Publication Abstract from PubMed

Regulation of myosin and filamentous actin interaction by tropomyosin is a central feature of contractile events in muscle and nonmuscle cells. However, little is known about molecular interactions within the complex and the trajectory of tropomyosin movement between its "open" and "closed" positions on the actin filament. Here, we report the 8 A resolution structure of the rigor (nucleotide-free) actin-tropomyosin-myosin complex determined by cryo-electron microscopy. The pseudoatomic model of the complex, obtained from fitting crystal structures into the map, defines the large interface involving two adjacent actin monomers and one tropomyosin pseudorepeat per myosin contact. Severe forms of hereditary myopathies are linked to mutations that critically perturb this interface. Myosin binding results in a 23 A shift of tropomyosin along actin. Complex domain motions occur in myosin, but not in actin. Based on our results, we propose a structural model for the tropomyosin-dependent modulation of myosin binding to actin.

Structure of the rigor actin-tropomyosin-Myosin complex.,Behrmann E, Muller M, Penczek PA, Mannherz HG, Manstein DJ, Raunser S Cell. 2012 Jul 20;150(2):327-38. PMID:22817895^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Behrmann E, Muller M, Penczek PA, Mannherz HG, Manstein DJ, Raunser S. Structure of the rigor actin-tropomyosin-Myosin complex. Cell. 2012 Jul 20;150(2):327-38. PMID:22817895 doi:10.1016/j.cell.2012.05.037

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4a7h"

@@ Line 3: / Line 3: @@
 <SX load='4a7h' size='340' side='right' viewer='molstar' caption='[[4a7h]], [[Resolution|resolution]] 7.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4a7h]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicdi Dicdi], [http://en.wikipedia.org/wiki/European_rabbit European rabbit] and [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A7H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A7H FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4a7h]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicdi Dicdi], [http://en.wikipedia.org/wiki/European_rabbit European rabbit] and [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A7H OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4A7H FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1alm|1alm]], [[1atn|1atn]], [[1eqy|1eqy]], [[1esv|1esv]], [[1h1v|1h1v]], [[1ijj|1ijj]], [[1j6z|1j6z]], [[1kxp|1kxp]], [[1lcu|1lcu]], [[1lkx|1lkx]], [[1lot|1lot]], [[1m8q|1m8q]], [[1ma9|1ma9]], [[1mvw|1mvw]], [[1nwk|1nwk]], [[1o18|1o18]], [[1o19|1o19]], [[1o1a|1o1a]], [[1o1b|1o1b]], [[1o1c|1o1c]], [[1o1d|1o1d]], [[1o1e|1o1e]], [[1o1f|1o1f]], [[1o1g|1o1g]], [[1p8z|1p8z]], [[1qz5|1qz5]], [[1qz6|1qz6]], [[1rdw|1rdw]], [[1rfq|1rfq]], [[1rgi|1rgi]], [[1s22|1s22]], [[1sqk|1sqk]], [[1t44|1t44]], [[1uy5|1uy5]], [[1wua|1wua]], [[1y64|1y64]], [[2a3z|2a3z]], [[2a40|2a40]], [[2a41|2a41]], [[2a42|2a42]], [[2a5x|2a5x]], [[2asm|2asm]], [[2aso|2aso]], [[2asp|2asp]], [[2d1k|2d1k]], [[2d3e|2d3e]], [[2ff3|2ff3]], [[2ff6|2ff6]], [[2fxu|2fxu]], [[2tma|2tma]], [[2v51|2v51]], [[2v52|2v52]], [[2vcp|2vcp]], [[2vyp|2vyp]], [[2w49|2w49]], [[2w4u|2w4u]], [[2y83|2y83]], [[2yje|2yje]], [[2yjf|2yjf]], [[4a7l|4a7l]], [[4a7n|4a7n]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Myosin_ATPase Myosin ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.1 3.6.4.1] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a7h OCA], [http://pdbe.org/4a7h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a7h RCSB], [http://www.ebi.ac.uk/pdbsum/4a7h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a7h ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4a7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a7h OCA], [http://pdbe.org/4a7h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a7h RCSB], [http://www.ebi.ac.uk/pdbsum/4a7h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a7h ProSAT]</span></td></tr>
 </table>
 == Function ==

4a7h

From Proteopedia

Revision as of 18:46, 10 April 2020

Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 2)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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