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4bkk

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Revision as of 18:54, 10 April 2020

The Respiratory Syncytial Virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid.

4bkk

Structural highlights

4bkk is a 24 chain structure with sequence from [1] and Hrsvl. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NCAP_HRSVA] Encapsidates the genome, protecting it from nucleases. The nucleocapsid (NC) has a helical structure. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases.^[1]

Publication Abstract from PubMed

Respiratory syncytial virus (RSV) is an important human pathogen. Its nucleocapsid (NC), which comprises the negative sense RNA viral genome coated by the viral nucleoprotein N, is a critical assembly that serves as template for both mRNA synthesis and genome replication. We have previously described the X-ray structure of an NC-like structure: a decameric ring formed of N-RNA that mimics one turn of the helical NC. In the absence of experimental data we had hypothesized that the NC helix would be right-handed, as the N-N contacts in the ring appeared to more easily adapt to that conformation. We now unambiguously show that the RSV NC is a left-handed helix. We further show that the contacts in the ring can be distorted to maintain key N-N-protein interactions in a left-handed helix, and discuss the implications of the resulting atomic model of the helical NC for viral replication and transcription.

The respiratory syncytial virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid.,Bakker SE, Duquerroy S, Galloux M, Loney C, Conner E, Eleouet JF, Rey FA, Bhella D J Gen Virol. 2013 Aug;94(Pt 8):1734-8. doi: 10.1099/vir.0.053025-0. Epub 2013 May, 15. PMID:23677789^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fearns R, Peeples ME, Collins PL. Increased expression of the N protein of respiratory syncytial virus stimulates minigenome replication but does not alter the balance between the synthesis of mRNA and antigenome. Virology. 1997 Sep 15;236(1):188-201. PMID:9299631 doi:10.1006/viro.1997.8734
↑ Bakker SE, Duquerroy S, Galloux M, Loney C, Conner E, Eleouet JF, Rey FA, Bhella D. The respiratory syncytial virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid. J Gen Virol. 2013 Aug;94(Pt 8):1734-8. doi: 10.1099/vir.0.053025-0. Epub 2013 May, 15. PMID:23677789 doi:http://dx.doi.org/10.1099/vir.0.053025-0

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4bkk"

@@ Line 3: / Line 3: @@
 <SX load='4bkk' size='340' side='right' viewer='molstar' caption='[[4bkk]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4bkk]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Hrsvl Hrsvl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BKK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BKK FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4bkk]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Hrsvl Hrsvl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BKK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4BKK FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bkk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bkk OCA], [http://pdbe.org/4bkk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bkk RCSB], [http://www.ebi.ac.uk/pdbsum/4bkk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bkk ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4bkk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bkk OCA], [http://pdbe.org/4bkk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bkk RCSB], [http://www.ebi.ac.uk/pdbsum/4bkk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bkk ProSAT]</span></td></tr>
 </table>
 == Function ==

4bkk

From Proteopedia

Revision as of 18:54, 10 April 2020

The Respiratory Syncytial Virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid.

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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