1azf

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[[Image:1azf.gif|left|200px]]
[[Image:1azf.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1azf |SIZE=350|CAPTION= <scene name='initialview01'>1azf</scene>, resolution 1.8&Aring;
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The line below this paragraph, containing "STRUCTURE_1azf", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1azf| PDB=1azf | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1azf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1azf OCA], [http://www.ebi.ac.uk/pdbsum/1azf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1azf RCSB]</span>
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}}
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'''CHICKEN EGG WHITE LYSOZYME CRYSTAL GROWN IN BROMIDE SOLUTION'''
'''CHICKEN EGG WHITE LYSOZYME CRYSTAL GROWN IN BROMIDE SOLUTION'''
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[[Category: Nadarajah, A.]]
[[Category: Nadarajah, A.]]
[[Category: Pusey, M L.]]
[[Category: Pusey, M L.]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: o-glycosyl hydrolase]]
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[[Category: O-glycosyl hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:52:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:50:30 2008''
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Revision as of 07:52, 2 May 2008

Image:1azf.gif

Template:STRUCTURE 1azf

CHICKEN EGG WHITE LYSOZYME CRYSTAL GROWN IN BROMIDE SOLUTION


Overview

Anions have been shown to play a dominant role in the crystallization of chicken egg-white lysozyme from salt solutions. Previous studies employing X-ray crystallography have found one chloride ion binding site in the tetragonal crystal form of the protein and four nitrate ion binding sites in the monoclinic form. In this study the anion positions in the tetragonal form were determined from the difference Fourier map obtained from lysozyme crystals grown in bromide and chloride solutions. Five possible anion-binding sites were found in this manner. Some of these sites were in pockets containing basic residues while others were near neutral, but polar, residues. The sole chloride ion binding site found in previous studies was confirmed, while four further sites were found which corresponded to the four binding sites found for nitrate ions in monoclinic crystals. The study suggests that most of the anion-binding sites in lysozyme remain unchanged even when different anions and different crystal forms of lysozyme are employed.

About this Structure

1AZF is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

Locations of bromide ions in tetragonal lysozyme crystals., Lim K, Nadarajah A, Forsythe EL, Pusey ML, Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):899-904. PMID:9757106 Page seeded by OCA on Fri May 2 10:52:31 2008

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