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4v7e

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Revision as of 19:30, 10 April 2020

Model of the small subunit RNA based on a 5.5 A cryo-EM map of Triticum aestivum translating 80S ribosome

4v7e, resolution 5.50Å

Structural highlights

4v7e is a 88 chain structure with sequence from Triticum aestivum. This structure supersedes the now removed PDB entries 3j5z, 3j60, 3j61, 3j62, 3iz6, 3iz7, 3iz9, 3izr and 3iz5. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The biogenesis of secretory as well as transmembrane proteins requires the activity of the universally conserved protein-conducting channel (PCC), the Sec61 complex (SecY complex in bacteria). In eukaryotic cells the PCC is located in the membrane of the endoplasmic reticulum where it can bind to translating ribosomes for co-translational protein transport. The Sec complex consists of three subunits (Sec61alpha, beta and gamma) and provides an aqueous environment for the translocation of hydrophilic peptides as well as a lateral opening in the Sec61alpha subunit that has been proposed to act as a gate for the membrane partitioning of hydrophobic domains. A plug helix and a so-called pore ring are believed to seal the PCC against ion flow and are proposed to rearrange for accommodation of translocating peptides. Several crystal and cryo-electron microscopy structures revealed different conformations of closed and partially open Sec61 and SecY complexes. However, in none of these samples has the translocation state been unambiguously defined biochemically. Here we present cryo-electron microscopy structures of ribosome-bound Sec61 complexes engaged in translocation or membrane insertion of nascent peptides. Our data show that a hydrophilic peptide can translocate through the Sec complex with an essentially closed lateral gate and an only slightly rearranged central channel. Membrane insertion of a hydrophobic domain seems to occur with the Sec complex opening the proposed lateral gate while rearranging the plug to maintain an ion permeability barrier. Taken together, we provide a structural model for the basic activities of the Sec61 complex as a protein-conducting channel.

Structures of the Sec61 complex engaged in nascent peptide translocation or membrane insertion.,Gogala M, Becker T, Beatrix B, Armache JP, Barrio-Garcia C, Berninghausen O, Beckmann R Nature. 2014 Feb 6;506(7486):107-10. doi: 10.1038/nature12950. PMID:24499919^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gogala M, Becker T, Beatrix B, Armache JP, Barrio-Garcia C, Berninghausen O, Beckmann R. Structures of the Sec61 complex engaged in nascent peptide translocation or membrane insertion. Nature. 2014 Feb 6;506(7486):107-10. doi: 10.1038/nature12950. PMID:24499919 doi:http://dx.doi.org/10.1038/nature12950

1 Structural highlights
2 Publication Abstract from PubMed
3 See Also
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4v7e"

@@ Line 3: / Line 3: @@
 <SX load='4v7e' size='340' side='right' viewer='molstar' caption='[[4v7e]], [[Resolution|resolution]] 5.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4v7e]] is a 88 chain structure with sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3j5z 3j5z], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3j60 3j60], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3j61 3j61], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3j62 3j62], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3iz6 3iz6], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3iz7 3iz7], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3iz9 3iz9], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3izr 3izr] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3iz5 3iz5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V7E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4V7E FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4v7e]] is a 88 chain structure with sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3j5z 3j5z], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3j60 3j60], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3j61 3j61], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3j62 3j62], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3iz6 3iz6], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3iz7 3iz7], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3iz9 3iz9], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3izr 3izr] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3iz5 3iz5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V7E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4V7E FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v7e OCA], [http://pdbe.org/4v7e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4v7e RCSB], [http://www.ebi.ac.uk/pdbsum/4v7e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4v7e ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4v7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v7e OCA], [http://pdbe.org/4v7e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4v7e RCSB], [http://www.ebi.ac.uk/pdbsum/4v7e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4v7e ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">

4v7e

From Proteopedia

Revision as of 19:30, 10 April 2020

Model of the small subunit RNA based on a 5.5 A cryo-EM map of Triticum aestivum translating 80S ribosome

Structural highlights

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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