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5hx2

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Revision as of 20:12, 10 April 2020

In vitro assembled star-shaped hubless T4 baseplate

5hx2, resolution 3.80Å

Structural highlights

5hx2 is a 9 chain structure with sequence from Bpt4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	7 (BPT4), 8 (BPT4), 6 (BPT4), 53 (BPT4), 10 (BPT4)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[BP53_BPT4] Baseplate protein that is part of the baseplate wedge (PubMed:15315755). Involved in the tail assembly.^[1] ^[2] [BP07_BPT4] Baseplate protein that is part of the baseplate wedge. Involved in the tail assembly.^[3] ^[4] [BP06_BPT4] Baseplate protein that is part of the baseplate wedge (PubMed:15315755). Involved in the tail assembly.^[5] ^[6] [BP08_BPT4] Baseplate protein that is part of the baseplate wedge. Involved in the tail assembly.^[7] ^[8] [BP10_BPT4] Baseplate protein that is part of the baseplate wedge and that connects the short tail fibers to the baseplate (PubMed:16554069). During infection, the baseplate undergoes a conformational change from a dome-shaped to a star-shaped structure. At this point, gp10 rotates and acts as a lever that unfolds the short tail fibers, which then interact with host cell surface receptors. Involved in the tail assembly.^[9] ^[10]

Publication Abstract from PubMed

Bacteriophage T4 consists of a head for protecting its genome and a sheathed tail for inserting its genome into a host. The tail terminates with a multiprotein baseplate that changes its conformation from a "high-energy" dome-shaped to a "low-energy" star-shaped structure during infection. Although these two structures represent different minima in the total energy landscape of the baseplate assembly, as the dome-shaped structure readily changes to the star-shaped structure when the virus infects a host bacterium, the dome-shaped structure must have more energy than the star-shaped structure. Here we describe the electron microscopy structure of a 3.3-MDa in vitro-assembled star-shaped baseplate with a resolution of 3.8 A. This structure, together with other genetic and structural data, shows why the high-energy baseplate is formed in the presence of the central hub and how the baseplate changes to the low-energy structure, via two steps during infection. Thus, the presence of the central hub is required to initiate the assembly of metastable, high-energy structures. If the high-energy structure is formed and stabilized faster than the low-energy structure, there will be insufficient components to assemble the low-energy structure.

Role of bacteriophage T4 baseplate in regulating assembly and infection.,Yap ML, Klose T, Arisaka F, Speir JA, Veesler D, Fokine A, Rossmann MG Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2654-9. doi:, 10.1073/pnas.1601654113. Epub 2016 Feb 29. PMID:26929357^[11]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Leiman PG, Chipman PR, Kostyuchenko VA, Mesyanzhinov VV, Rossmann MG. Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host. Cell. 2004 Aug 20;118(4):419-29. PMID:15315755 doi:10.1016/j.cell.2004.07.022
↑ Leiman PG, Arisaka F, van Raaij MJ, Kostyuchenko VA, Aksyuk AA, Kanamaru S, Rossmann MG. Morphogenesis of the T4 tail and tail fibers. Virol J. 2010 Dec 3;7:355. doi: 10.1186/1743-422X-7-355. PMID:21129200 doi:10.1186/1743-422X-7-355
↑ Watts NR, Hainfeld J, Coombs DH. Localization of the proteins gp7, gp8 and gp10 in the bacteriophage T4 baseplate with colloidal gold: F(ab)2 and undecagold: Fab' conjugates. J Mol Biol. 1990 Nov 20;216(2):315-25. PMID:2254933 doi:http://dx.doi.org/10.1016/S0022-2836(05)80323-7
↑ Leiman PG, Arisaka F, van Raaij MJ, Kostyuchenko VA, Aksyuk AA, Kanamaru S, Rossmann MG. Morphogenesis of the T4 tail and tail fibers. Virol J. 2010 Dec 3;7:355. doi: 10.1186/1743-422X-7-355. PMID:21129200 doi:10.1186/1743-422X-7-355
↑ Leiman PG, Chipman PR, Kostyuchenko VA, Mesyanzhinov VV, Rossmann MG. Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host. Cell. 2004 Aug 20;118(4):419-29. PMID:15315755 doi:10.1016/j.cell.2004.07.022
↑ Leiman PG, Arisaka F, van Raaij MJ, Kostyuchenko VA, Aksyuk AA, Kanamaru S, Rossmann MG. Morphogenesis of the T4 tail and tail fibers. Virol J. 2010 Dec 3;7:355. doi: 10.1186/1743-422X-7-355. PMID:21129200 doi:10.1186/1743-422X-7-355
↑ Leiman PG, Chipman PR, Kostyuchenko VA, Mesyanzhinov VV, Rossmann MG. Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host. Cell. 2004 Aug 20;118(4):419-29. PMID:15315755 doi:10.1016/j.cell.2004.07.022
↑ Leiman PG, Arisaka F, van Raaij MJ, Kostyuchenko VA, Aksyuk AA, Kanamaru S, Rossmann MG. Morphogenesis of the T4 tail and tail fibers. Virol J. 2010 Dec 3;7:355. doi: 10.1186/1743-422X-7-355. PMID:21129200 doi:10.1186/1743-422X-7-355
↑ Leiman PG, Shneider MM, Mesyanzhinov VV, Rossmann MG. Evolution of bacteriophage tails: Structure of T4 gene product 10. J Mol Biol. 2006 May 5;358(3):912-21. Epub 2006 Mar 9. PMID:16554069 doi:10.1016/j.jmb.2006.02.058
↑ Leiman PG, Arisaka F, van Raaij MJ, Kostyuchenko VA, Aksyuk AA, Kanamaru S, Rossmann MG. Morphogenesis of the T4 tail and tail fibers. Virol J. 2010 Dec 3;7:355. doi: 10.1186/1743-422X-7-355. PMID:21129200 doi:10.1186/1743-422X-7-355
↑ Yap ML, Klose T, Arisaka F, Speir JA, Veesler D, Fokine A, Rossmann MG. Role of bacteriophage T4 baseplate in regulating assembly and infection. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2654-9. doi:, 10.1073/pnas.1601654113. Epub 2016 Feb 29. PMID:26929357 doi:http://dx.doi.org/10.1073/pnas.1601654113

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5hx2"

@@ Line 3: / Line 3: @@
 <SX load='5hx2' size='340' side='right' viewer='molstar' caption='[[5hx2]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5hx2]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HX2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HX2 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5hx2]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HX2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HX2 FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4]), 8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4]), 6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4]), 53 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4]), 10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hx2 OCA], [http://pdbe.org/5hx2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hx2 RCSB], [http://www.ebi.ac.uk/pdbsum/5hx2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hx2 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hx2 OCA], [http://pdbe.org/5hx2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hx2 RCSB], [http://www.ebi.ac.uk/pdbsum/5hx2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hx2 ProSAT]</span></td></tr>
 </table>
 == Function ==

5hx2

From Proteopedia

Revision as of 20:12, 10 April 2020

In vitro assembled star-shaped hubless T4 baseplate

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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