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5jpq

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Revision as of 20:23, 10 April 2020

Cryo-EM structure of the 90S pre-ribosome

5jpq, resolution 7.30Å

Structural highlights

5jpq is a 55 chain structure with sequence from Baker's yeast, Chaetomium thermophilum and Chaetomium thermophilum . Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[G0S3V7_CHATD] Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.[PIRNR:PIRNR006515] [UTP21_YEAST] Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.^[1]

Publication Abstract from PubMed

The 90S pre-ribosome is an early biogenesis intermediate formed during co-transcriptional ribosome formation, composed of approximately 70 assembly factors and several small nucleolar RNAs (snoRNAs) that associate with nascent pre-rRNA. We report the cryo-EM structure of the Chaetomium thermophilum 90S pre-ribosome, revealing how a network of biogenesis factors including 19 beta-propellers and large alpha-solenoid proteins engulfs the pre-rRNA. Within the 90S pre-ribosome, we identify the UTP-A, UTP-B, Mpp10-Imp3-Imp4, Bms1-Rcl1, and U3 snoRNP modules, which are organized around 5'-ETS and partially folded 18S rRNA. The U3 snoRNP is strategically positioned at the center of the 90S particle to perform its multiple tasks during pre-rRNA folding and processing. The architecture of the elusive 90S pre-ribosome gives unprecedented structural insight into the early steps of pre-rRNA maturation. Nascent rRNA that is co-transcriptionally folded and given a particular shape by encapsulation within a dedicated mold-like structure is reminiscent of how polypeptides use chaperone chambers for their protein folding.

Architecture of the 90S Pre-ribosome: A Structural View on the Birth of the Eukaryotic Ribosome.,Kornprobst M, Turk M, Kellner N, Cheng J, Flemming D, Kos-Braun I, Kos M, Thoms M, Berninghausen O, Beckmann R, Hurt E Cell. 2016 Jul 14;166(2):380-93. doi: 10.1016/j.cell.2016.06.014. PMID:27419870^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bernstein KA, Gallagher JE, Mitchell BM, Granneman S, Baserga SJ. The small-subunit processome is a ribosome assembly intermediate. Eukaryot Cell. 2004 Dec;3(6):1619-26. PMID:15590835 doi:http://dx.doi.org/10.1128/EC.3.6.1619-1626.2004
↑ Kornprobst M, Turk M, Kellner N, Cheng J, Flemming D, Kos-Braun I, Kos M, Thoms M, Berninghausen O, Beckmann R, Hurt E. Architecture of the 90S Pre-ribosome: A Structural View on the Birth of the Eukaryotic Ribosome. Cell. 2016 Jul 14;166(2):380-93. doi: 10.1016/j.cell.2016.06.014. PMID:27419870 doi:http://dx.doi.org/10.1016/j.cell.2016.06.014

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5jpq"

@@ Line 3: / Line 3: @@
 <SX load='5jpq' size='340' side='right' viewer='molstar' caption='[[5jpq]], [[Resolution|resolution]] 7.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5jpq]] is a 55 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast], [http://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum] and [http://en.wikipedia.org/wiki/Chaetomium_thermophilum_ Chaetomium thermophilum ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JPQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JPQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5jpq]] is a 55 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast], [http://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum] and [http://en.wikipedia.org/wiki/Chaetomium_thermophilum_ Chaetomium thermophilum ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JPQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JPQ FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jpq OCA], [http://pdbe.org/5jpq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jpq RCSB], [http://www.ebi.ac.uk/pdbsum/5jpq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jpq ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jpq OCA], [http://pdbe.org/5jpq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jpq RCSB], [http://www.ebi.ac.uk/pdbsum/5jpq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jpq ProSAT]</span></td></tr>
 </table>
 == Function ==

5jpq

From Proteopedia

Revision as of 20:23, 10 April 2020

Cryo-EM structure of the 90S pre-ribosome

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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