1azo

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[[Image:1azo.jpg|left|200px]]
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{{Structure
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1azo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1azo OCA], [http://www.ebi.ac.uk/pdbsum/1azo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1azo RCSB]</span>
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'''DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI'''
'''DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI'''
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[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Yang, W.]]
[[Category: Yang, W.]]
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[[Category: dna repair]]
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[[Category: Dna repair]]
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[[Category: endonuclease]]
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[[Category: Endonuclease]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: muth]]
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[[Category: Muth]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:53:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:50:43 2008''
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Revision as of 07:53, 2 May 2008

Image:1azo.jpg

Template:STRUCTURE 1azo

DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI


Overview

MutS, MutL and MutH are the three essential proteins for initiation of methyl-directed DNA mismatch repair to correct mistakes made during DNA replication in Escherichia coli. MutH cleaves a newly synthesized and unmethylated daughter strand 5' to the sequence d(GATC) in a hemi-methylated duplex. Activation of MutH requires the recognition of a DNA mismatch by MutS and MutL. We have crystallized MutH in two space groups and solved the structures at 1.7 and 2.3 A resolution, respectively. The active site of MutH is located at an interface between two subdomains that pivot relative to one another, as revealed by comparison of the crystal structures, and this presumably regulates the nuclease activity. The relative motion of the two subdomains in MutH correlates with the position of a protruding C-terminal helix. This helix appears to act as a molecular lever through which MutS and MutL may communicate the detection of a DNA mismatch and activate MutH. With sequence homology to Sau3AI and structural similarity to PvuII endonuclease, MutH is clearly related to these enzymes by divergent evolution, and this suggests that type II restriction endonucleases evolved from a common ancestor.

About this Structure

1AZO is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleases., Ban C, Yang W, EMBO J. 1998 Mar 2;17(5):1526-34. PMID:9482749 Page seeded by OCA on Fri May 2 10:53:09 2008

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