This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

5xsy

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Structure of the Nav1.4-beta1 complex from electric eel

5xsy, resolution 4.00Å

Structural highlights

5xsy is a 2 chain structure with sequence from Electrophorus electricus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SCNA_ELEEL] Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.^[1]

Publication Abstract from PubMed

Voltage-gated sodium (Nav) channels initiate and propagate action potentials. Here, we present the cryo-EM structure of EeNav1.4, the Nav channel from electric eel, in complex with the beta1 subunit at 4.0 A resolution. The immunoglobulin domain of beta1 docks onto the extracellular L5I and L6IV loops of EeNav1.4 via extensive polar interactions, and the single transmembrane helix interacts with the third voltage-sensing domain (VSDIII). The VSDs exhibit "up" conformations, while the intracellular gate of the pore domain is kept open by a digitonin-like molecule. Structural comparison with closed NavPaS shows that the outward transfer of gating charges is coupled to the iris-like pore domain dilation through intricate force transmissions involving multiple channel segments. The IFM fast inactivation motif on the III-IV linker is plugged into the corner enclosed by the outer S4-S5 and inner S6 segments in repeats III and IV, suggesting a potential allosteric blocking mechanism for fast inactivation.

Structure of the Nav1.4-beta1 Complex from Electric Eel.,Yan Z, Zhou Q, Wang L, Wu J, Zhao Y, Huang G, Peng W, Shen H, Lei J, Yan N Cell. 2017 Jul 27;170(3):470-482.e11. doi: 10.1016/j.cell.2017.06.039. Epub 2017 , Jul 20. PMID:28735751^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Noda M, Numa S. Structure and function of sodium channel. J Recept Res. 1987;7(1-4):467-97. PMID:2442385
↑ Yan Z, Zhou Q, Wang L, Wu J, Zhao Y, Huang G, Peng W, Shen H, Lei J, Yan N. Structure of the Nav1.4-beta1 Complex from Electric Eel. Cell. 2017 Jul 27;170(3):470-482.e11. doi: 10.1016/j.cell.2017.06.039. Epub 2017 , Jul 20. PMID:28735751 doi:http://dx.doi.org/10.1016/j.cell.2017.06.039

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5xsy"

@@ Line 3: / Line 3: @@
 <SX load='5xsy' size='340' side='right' viewer='molstar' caption='[[5xsy]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5xsy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Electrophorus_electricus Electrophorus electricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XSY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XSY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5xsy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Electrophorus_electricus Electrophorus electricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XSY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XSY FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xsy OCA], [http://pdbe.org/5xsy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xsy RCSB], [http://www.ebi.ac.uk/pdbsum/5xsy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xsy ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xsy OCA], [http://pdbe.org/5xsy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xsy RCSB], [http://www.ebi.ac.uk/pdbsum/5xsy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xsy ProSAT]</span></td></tr>
 </table>
 == Function ==

5xsy

From Proteopedia

Current revision

Structure of the Nav1.4-beta1 complex from electric eel

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox