6d6v

From Proteopedia

(Difference between revisions)

Revision as of 23:57, 10 April 2020

CryoEM structure of Tetrahymena telomerase with telomeric DNA at 4.8 Angstrom resolution

6d6v, resolution 4.80Å

Structural highlights

6d6v is a 8 chain structure with sequence from Tetrahymena thermophila. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Activity:	RNA-directed DNA polymerase, with EC number 2.7.7.49
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TERT_TETTH] Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme (By similarity).

Publication Abstract from PubMed

Telomerase is an RNA-protein complex (RNP) that extends telomeric DNA at the 3' ends of chromosomes using its telomerase reverse transcriptase (TERT) and integral template-containing telomerase RNA (TER). Its activity is a critical determinant of human health, affecting aging, cancer, and stem cell renewal. Lack of atomic models of telomerase, particularly one with DNA bound, has limited our mechanistic understanding of telomeric DNA repeat synthesis. We report the 4.8 A resolution cryoelectron microscopy structure of active Tetrahymena telomerase bound to telomeric DNA. The catalytic core is an intricately interlocked structure of TERT and TER, including a previously structurally uncharacterized TERT domain that interacts with the TEN domain to physically enclose TER and regulate activity. This complete structure of a telomerase catalytic core and its interactions with telomeric DNA from the template to telomere-interacting p50-TEB complex provides unanticipated insights into telomerase assembly and catalytic cycle and a new paradigm for a reverse transcriptase RNP.

Structure of Telomerase with Telomeric DNA.,Jiang J, Wang Y, Susac L, Chan H, Basu R, Zhou ZH, Feigon J Cell. 2018 May 17;173(5):1179-1190.e13. doi: 10.1016/j.cell.2018.04.038. PMID:29775593^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jiang J, Wang Y, Susac L, Chan H, Basu R, Zhou ZH, Feigon J. Structure of Telomerase with Telomeric DNA. Cell. 2018 May 17;173(5):1179-1190.e13. doi: 10.1016/j.cell.2018.04.038. PMID:29775593 doi:http://dx.doi.org/10.1016/j.cell.2018.04.038

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6d6v"

@@ Line 3: / Line 3: @@
 <SX load='6d6v' size='340' side='right' viewer='molstar' caption='[[6d6v]], [[Resolution|resolution]] 4.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6d6v]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D6V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D6V FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6d6v]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D6V OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6D6V FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d6v OCA], [http://pdbe.org/6d6v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d6v RCSB], [http://www.ebi.ac.uk/pdbsum/6d6v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d6v ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6d6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d6v OCA], [http://pdbe.org/6d6v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d6v RCSB], [http://www.ebi.ac.uk/pdbsum/6d6v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d6v ProSAT]</span></td></tr>
 </table>
 == Function ==

6d6v

From Proteopedia

Revision as of 23:57, 10 April 2020

CryoEM structure of Tetrahymena telomerase with telomeric DNA at 4.8 Angstrom resolution

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox