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6ebl

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Revision as of 00:23, 11 April 2020

The voltage-activated Kv1.2-2.1 paddle chimera channel in lipid nanodiscs, cytosolic domain

6ebl, resolution 3.00Å

Structural highlights

6ebl is a 8 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[KCAB2_RAT] Accessory potassium channel protein which modulates the activity of the pore-forming alpha subunit. [KCNA2_RAT] Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.^[1]

Publication Abstract from PubMed

Voltage-activated potassium (Kv) channels open to conduct K(+) ions in response to membrane depolarization, and subsequently enter non-conducting states through distinct mechanisms of inactivation. X-ray structures of detergent-solubilized Kv channels appear to have captured an open state even though a non-conducting C-type inactivated state would predominate in membranes in the absence of a transmembrane voltage. However, structures for a voltage-activated ion channel in a lipid bilayer environment have not yet been reported. Here we report the structure of the Kv1.2-2.1 paddle chimera channel reconstituted into lipid nanodiscs using single-particle cryo-electron microscopy. At a resolution of ~3 A for the cytosolic domain and ~4 A for the transmembrane domain, the structure determined in nanodiscs is similar to the previously determined X-ray structure. Our findings show that large differences in structure between detergent and lipid bilayer environments are unlikely, and enable us to propose possible structural mechanisms for C-type inactivation.

Single-particle cryo-EM structure of a voltage-activated potassium channel in lipid nanodiscs.,Matthies D, Bae C, Toombes GE, Fox T, Bartesaghi A, Subramaniam S, Swartz KJ Elife. 2018 Aug 15;7. pii: 37558. doi: 10.7554/eLife.37558. PMID:30109985^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lev S, Moreno H, Martinez R, Canoll P, Peles E, Musacchio JM, Plowman GD, Rudy B, Schlessinger J. Protein tyrosine kinase PYK2 involved in Ca(2+)-induced regulation of ion channel and MAP kinase functions. Nature. 1995 Aug 31;376(6543):737-45. PMID:7544443 doi:http://dx.doi.org/10.1038/376737a0
↑ Matthies D, Bae C, Toombes GE, Fox T, Bartesaghi A, Subramaniam S, Swartz KJ. Single-particle cryo-EM structure of a voltage-activated potassium channel in lipid nanodiscs. Elife. 2018 Aug 15;7. pii: 37558. doi: 10.7554/eLife.37558. PMID:30109985 doi:http://dx.doi.org/10.7554/eLife.37558

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6ebl"

@@ Line 3: / Line 3: @@
 <SX load='6ebl' size='340' side='right' viewer='molstar' caption='[[6ebl]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6ebl]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EBL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6ebl]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EBL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6EBL FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ebl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ebl OCA], [http://pdbe.org/6ebl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ebl RCSB], [http://www.ebi.ac.uk/pdbsum/6ebl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ebl ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ebl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ebl OCA], [http://pdbe.org/6ebl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ebl RCSB], [http://www.ebi.ac.uk/pdbsum/6ebl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ebl ProSAT]</span></td></tr>
 </table>
 == Function ==

6ebl

From Proteopedia

Revision as of 00:23, 11 April 2020

The voltage-activated Kv1.2-2.1 paddle chimera channel in lipid nanodiscs, cytosolic domain

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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