6esg

From Proteopedia

(Difference between revisions)

Revision as of 00:32, 11 April 2020

Nucleosome breathing : Class 2

6esg, resolution 5.40Å

Structural highlights

6esg is a 10 chain structure with sequence from [1] and African clawed frog. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	hist1h2aj, LOC494591 (African clawed frog)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[H2B11_XENLA] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [H32_XENLA] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [H4_XENLA] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Publication Abstract from PubMed

Nucleosomes, the basic units of chromatin, package and regulate expression of eukaryotic genomes. Although the structure of the intact nucleosome is well characterized, little is known about structures of partially unwrapped, transient intermediates. In this study, we present nine cryo-EM structures of distinct conformations of nucleosome and subnucleosome particles. These structures show that initial DNA breathing induces conformational changes in the histone octamer, particularly in histone H3, that propagate through the nucleosome and prevent symmetrical DNA opening. Rearrangements in the H2A-H2B dimer strengthen interaction with the unwrapping DNA and promote nucleosome stability. In agreement with this, cross-linked H2A-H2B that cannot accommodate unwrapping of the DNA is not stably maintained in the nucleosome. H2A-H2B release and DNA unwrapping occur simultaneously, indicating that DNA is essential in stabilizing the dimer in the nucleosome. Our structures reveal intrinsic nucleosomal plasticity that is required for nucleosome stability and might be exploited by extrinsic protein factors.

Histone octamer rearranges to adapt to DNA unwrapping.,Bilokapic S, Strauss M, Halic M Nat Struct Mol Biol. 2018 Jan;25(1):101-108. doi: 10.1038/s41594-017-0005-5. Epub, 2017 Dec 11. PMID:29323273^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bilokapic S, Strauss M, Halic M. Histone octamer rearranges to adapt to DNA unwrapping. Nat Struct Mol Biol. 2018 Jan;25(1):101-108. doi: 10.1038/s41594-017-0005-5. Epub, 2017 Dec 11. PMID:29323273 doi:http://dx.doi.org/10.1038/s41594-017-0005-5

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6esg"

@@ Line 3: / Line 3: @@
 <SX load='6esg' size='340' side='right' viewer='molstar' caption='[[6esg]], [[Resolution|resolution]] 5.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6esg]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/African_clawed_frog African clawed frog]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ESG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ESG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6esg]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/African_clawed_frog African clawed frog]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ESG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ESG FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hist1h2aj, LOC494591 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6esg OCA], [http://pdbe.org/6esg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6esg RCSB], [http://www.ebi.ac.uk/pdbsum/6esg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6esg ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6esg OCA], [http://pdbe.org/6esg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6esg RCSB], [http://www.ebi.ac.uk/pdbsum/6esg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6esg ProSAT]</span></td></tr>
 </table>
 == Function ==

6esg

From Proteopedia

Revision as of 00:32, 11 April 2020

Nucleosome breathing : Class 2

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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