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6gfw

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Cryo-EM structure of bacterial RNA polymerase-sigma54 holoenzyme initial transcribing complex

6gfw, resolution 3.70Å

Structural highlights

6gfw is a 9 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Activity:	DNA-directed RNA polymerase, with EC number 2.7.7.6
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RPOC_ECOLI] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_01322] [A0A0J4U551_KLEPN] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.[PIRNR:PIRNR000774] [RPOB_ECOLI] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_01321] [RPOA_ECOLI] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.[HAMAP-Rule:MF_00059] [RPOZ_ECOLI] Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.[HAMAP-Rule:MF_00366]

Publication Abstract from PubMed

Gene transcription is carried out by multi-subunit RNA polymerases (RNAPs). Transcription initiation is a dynamic multi-step process that involves the opening of the double-stranded DNA to form a transcription bubble and delivery of the template strand deep into the RNAP for RNA synthesis. Applying cryoelectron microscopy to a unique transcription system using sigma(54) (sigma(N)), the major bacterial variant sigma factor, we capture a new intermediate state at 4.1 A where promoter DNA is caught at the entrance of the RNAP cleft. Combining with new structures of the open promoter complex and an initial de novo transcribing complex at 3.4 and 3.7 A, respectively, our studies reveal the dynamics of DNA loading and mechanism of transcription bubble stabilization that involves coordinated, large-scale conformational changes of the universally conserved features within RNAP and DNA. In addition, our studies reveal a novel mechanism of strand separation by sigma(54).

Structures of Bacterial RNA Polymerase Complexes Reveal the Mechanism of DNA Loading and Transcription Initiation.,Glyde R, Ye F, Jovanovic M, Kotta-Loizou I, Buck M, Zhang X Mol Cell. 2018 Jun 21;70(6):1111-1120.e3. doi: 10.1016/j.molcel.2018.05.021. PMID:29932903^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Glyde R, Ye F, Jovanovic M, Kotta-Loizou I, Buck M, Zhang X. Structures of Bacterial RNA Polymerase Complexes Reveal the Mechanism of DNA Loading and Transcription Initiation. Mol Cell. 2018 Jun 21;70(6):1111-1120.e3. doi: 10.1016/j.molcel.2018.05.021. PMID:29932903 doi:http://dx.doi.org/10.1016/j.molcel.2018.05.021

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6gfw"

@@ Line 3: / Line 3: @@
 <SX load='6gfw' size='340' side='right' viewer='molstar' caption='[[6gfw]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6gfw]] is a 9 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GFW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GFW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6gfw]] is a 9 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GFW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6GFW FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gfw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gfw OCA], [http://pdbe.org/6gfw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gfw RCSB], [http://www.ebi.ac.uk/pdbsum/6gfw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gfw ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6gfw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gfw OCA], [http://pdbe.org/6gfw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gfw RCSB], [http://www.ebi.ac.uk/pdbsum/6gfw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gfw ProSAT]</span></td></tr>
 </table>
 == Function ==

6gfw

From Proteopedia

Current revision

Cryo-EM structure of bacterial RNA polymerase-sigma54 holoenzyme initial transcribing complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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