6jt2

From Proteopedia

(Difference between revisions)

Revision as of 02:16, 11 April 2020

Structure of human soluble guanylate cyclase in the NO activated state

6jt2, resolution 3.80Å

Structural highlights

6jt2 is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	GUCY1A1, GUC1A3, GUCSA3, GUCY1A3 (HUMAN), GUCY1B1, GUC1B3, GUCSB3, GUCY1B3 (HUMAN)
Activity:	Guanylate cyclase, with EC number 4.6.1.2
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[GCYA1_HUMAN] Moyamoya disease with early-onset achalasia. The disease is caused by mutations affecting the gene represented in this entry.

Publication Abstract from PubMed

Soluble guanylate cyclase (sGC) is the primary sensor of nitric oxide. It has a central role in nitric oxide signalling and has been implicated in many essential physiological processes and disease conditions. The binding of nitric oxide boosts the enzymatic activity of sGC. However, the mechanism by which nitric oxide activates the enzyme is unclear. Here we report the cryo-electron microscopy structures of the human sGCalpha1beta1 heterodimer in different functional states. These structures revealed that the transducer module bridges the nitric oxide sensor module and the catalytic module. Binding of nitric oxide to the beta1 haem-nitric oxide and oxygen binding (H-NOX) domain triggers the structural rearrangement of the sensor module and a conformational switch of the transducer module from bending to straightening. The resulting movement of the N termini of the catalytic domains drives structural changes within the catalytic module, which in turn boost the enzymatic activity of sGC.

Structural insights into the mechanism of human soluble guanylate cyclase.,Kang Y, Liu R, Wu JX, Chen L Nature. 2019 Oct;574(7777):206-210. doi: 10.1038/s41586-019-1584-6. Epub 2019 Sep, 12. PMID:31514202^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kang Y, Liu R, Wu JX, Chen L. Structural insights into the mechanism of human soluble guanylate cyclase. Nature. 2019 Oct;574(7777):206-210. doi: 10.1038/s41586-019-1584-6. Epub 2019 Sep, 12. PMID:31514202 doi:http://dx.doi.org/10.1038/s41586-019-1584-6

1 Structural highlights
2 Disease
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6jt2"

@@ Line 3: / Line 3: @@
 <SX load='6jt2' size='340' side='right' viewer='molstar' caption='[[6jt2]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6jt2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JT2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JT2 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6jt2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JT2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6JT2 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GUCY1A1, GUC1A3, GUCSA3, GUCY1A3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), GUCY1B1, GUC1B3, GUCSB3, GUCY1B3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Guanylate_cyclase Guanylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.2 4.6.1.2] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jt2 OCA], [http://pdbe.org/6jt2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jt2 RCSB], [http://www.ebi.ac.uk/pdbsum/6jt2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jt2 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6jt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jt2 OCA], [http://pdbe.org/6jt2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jt2 RCSB], [http://www.ebi.ac.uk/pdbsum/6jt2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jt2 ProSAT]</span></td></tr>
 </table>
 == Disease ==

6jt2

From Proteopedia

Revision as of 02:16, 11 April 2020

Structure of human soluble guanylate cyclase in the NO activated state

Structural highlights

Disease

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox