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6nmd

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Revision as of 03:26, 11 April 2020

cryo-EM Structure of the LbCas12a-crRNA-AcrVA1 complex

6nmd, resolution 3.49Å

Structural highlights

6nmd is a 3 chain structure with sequence from Atcc baa-1259 and Lachnospiraceae bacterium nd2006. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	AAX09_07405 (ATCC BAA-1259)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

CRISPR-Cas12a (Cpf1), a type V CRISPR-associated nuclease, provides bacterial immunity against bacteriophages and plasmids but also serves as a tool for genome editing. Foreign nucleic acids are integrated into the CRISPR locus, prompting transcription of CRISPR RNAs (crRNAs) that guide Cas12a cleavage of foreign complementary DNA. However, mobile genetic elements counteract Cas12a with inhibitors, notably type V-A anti-CRISPRs (AcrVAs). We present cryoelectron microscopy structures of Cas12a-crRNA bound to AcrVA1 and AcrVA4 at 3.5 and 3.3 A resolutions, respectively. AcrVA1 is sandwiched between the recognition (REC) and nuclease (NUC) lobes of Cas12a and inserts into the binding pocket for the protospacer-adjacent motif (PAM), a short DNA sequence guiding Cas12a targeting. AcrVA1 cleaves crRNA in a Cas12a-dependent manner, inactivating Cas12a-crRNA complexes. The AcrVA4 dimer is anchored around the crRNA pseudoknot of Cas12a-crRNA, preventing required conformational changes for crRNA-DNA heteroduplex formation. These results uncover molecular mechanisms for CRISPR-Cas12a inhibition, providing insights into bacteria-phage dynamics.

Structural Basis for the Inhibition of CRISPR-Cas12a by Anti-CRISPR Proteins.,Zhang H, Li Z, Daczkowski CM, Gabel C, Mesecar AD, Chang L Cell Host Microbe. 2019 May 27. pii: S1931-3128(19)30249-5. doi:, 10.1016/j.chom.2019.05.004. PMID:31155345^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhang H, Li Z, Daczkowski CM, Gabel C, Mesecar AD, Chang L. Structural Basis for the Inhibition of CRISPR-Cas12a by Anti-CRISPR Proteins. Cell Host Microbe. 2019 May 27. pii: S1931-3128(19)30249-5. doi:, 10.1016/j.chom.2019.05.004. PMID:31155345 doi:http://dx.doi.org/10.1016/j.chom.2019.05.004

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6nmd"

@@ Line 3: / Line 3: @@
 <SX load='6nmd' size='340' side='right' viewer='molstar' caption='[[6nmd]], [[Resolution|resolution]] 3.49&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6nmd]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_baa-1259 Atcc baa-1259] and [http://en.wikipedia.org/wiki/Lachnospiraceae_bacterium_nd2006 Lachnospiraceae bacterium nd2006]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NMD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NMD FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6nmd]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_baa-1259 Atcc baa-1259] and [http://en.wikipedia.org/wiki/Lachnospiraceae_bacterium_nd2006 Lachnospiraceae bacterium nd2006]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NMD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NMD FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AAX09_07405 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=386891 ATCC BAA-1259])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nmd OCA], [http://pdbe.org/6nmd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nmd RCSB], [http://www.ebi.ac.uk/pdbsum/6nmd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nmd ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nmd OCA], [http://pdbe.org/6nmd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nmd RCSB], [http://www.ebi.ac.uk/pdbsum/6nmd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nmd ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">

6nmd

From Proteopedia

Revision as of 03:26, 11 April 2020

cryo-EM Structure of the LbCas12a-crRNA-AcrVA1 complex

Structural highlights

Publication Abstract from PubMed

References

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