6psy

From Proteopedia

(Difference between revisions)

Revision as of 04:35, 11 April 2020

Cryo-EM structure of S. cerevisiae Drs2p-Cdc50p in the autoinhibited apo form

6psy, resolution 2.80Å

Structural highlights

6psy is a 2 chain structure with sequence from Saccharomyces cerevisiae w303. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	DRS2, YAL026C, FUN38 (Saccharomyces cerevisiae W303), CDC50, YCR094W, YCR94W (Saccharomyces cerevisiae W303)
Activity:	P-type phospholipid transporter, with EC number 7.6.2.1
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ATC3_YEAST] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids (Potential). Seems to be involved in ribosome assembly. [CDC50_YEAST] Required for polarized cell growth.

Publication Abstract from PubMed

The heterodimeric eukaryotic Drs2p-Cdc50p complex is a lipid flippase that maintains cell membrane asymmetry. The enzyme complex exists in an autoinhibited form in the absence of an activator and is specifically activated by phosphatidylinositol-4-phosphate (PI4P), although the underlying mechanisms have been unclear. Here we report the cryo-EM structures of intact Drs2p-Cdc50p isolated from S. cerevisiae in apo form and in the PI4P-activated form at 2.8 A and 3.3 A resolution, respectively. The structures reveal that the Drs2p C-terminus lines a long groove in the cytosolic regulatory region to inhibit the flippase activity. PIP4 binding in a cytosol-proximal membrane region triggers a 90 degrees rotation of a cytosolic helix switch that is located just upstream of the inhibitory C-terminal peptide. The rotation of the helix switch dislodges the C-terminus from the regulatory region, activating the flippase.

Autoinhibition and activation mechanisms of the eukaryotic lipid flippase Drs2p-Cdc50p.,Bai L, Kovach A, You Q, Hsu HC, Zhao G, Li H Nat Commun. 2019 Sep 12;10(1):4142. doi: 10.1038/s41467-019-12191-9. PMID:31515475^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bai L, Kovach A, You Q, Hsu HC, Zhao G, Li H. Autoinhibition and activation mechanisms of the eukaryotic lipid flippase Drs2p-Cdc50p. Nat Commun. 2019 Sep 12;10(1):4142. doi: 10.1038/s41467-019-12191-9. PMID:31515475 doi:http://dx.doi.org/10.1038/s41467-019-12191-9

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6psy"

@@ Line 3: / Line 3: @@
 <SX load='6psy' size='340' side='right' viewer='molstar' caption='[[6psy]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6psy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_w303 Saccharomyces cerevisiae w303]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PSY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PSY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6psy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_w303 Saccharomyces cerevisiae w303]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PSY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6PSY FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DRS2, YAL026C, FUN38 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=580240 Saccharomyces cerevisiae W303]), CDC50, YCR094W, YCR94W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=580240 Saccharomyces cerevisiae W303])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/P-type_phospholipid_transporter P-type phospholipid transporter], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.6.2.1 7.6.2.1] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6psy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6psy OCA], [http://pdbe.org/6psy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6psy RCSB], [http://www.ebi.ac.uk/pdbsum/6psy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6psy ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6psy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6psy OCA], [http://pdbe.org/6psy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6psy RCSB], [http://www.ebi.ac.uk/pdbsum/6psy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6psy ProSAT]</span></td></tr>
 </table>
 == Function ==

6psy

From Proteopedia

Revision as of 04:35, 11 April 2020

Cryo-EM structure of S. cerevisiae Drs2p-Cdc50p in the autoinhibited apo form

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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