6qle

From Proteopedia

(Difference between revisions)

Current revision

Structure of inner kinetochore CCAN complex

6qle, resolution 3.55Å

Structural highlights

6qle is a 11 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	FOSTERSB_4937 (ATCC 18824), NKP1, YDR383C (ATCC 18824), NKP2, YLR315W (ATCC 18824), SCRG_04321 (ATCC 18824), MCM22, YJR135C, J2122 (ATCC 18824), IML3, MCM19, YBR107C, YBR0836 (ATCC 18824), CHL4, CTF17, MCM17, YDR254W, YD9320A.04 (ATCC 18824), MCM21, CTF5, YDR318W (ATCC 18824), CTF19, MCM18, YPL018W, LPB13W (ATCC 18824), OKP1, YGR179C (ATCC 18824), AME1, ARP100, YBR211C, YBR1458 (ATCC 18824)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CENPO_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore COMA complex, which connects centromere-associated proteins and the outer kinetochore. COMA interacts with other inner kinetochore proteins to form the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.^[1] [NKP2_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.^[2] [CENPK_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.^[3] [CENPP_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore COMA complex, which connects centromere-associated proteins and the outer kinetochore. COMA interacts with other inner kinetochore proteins to form the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.^[4] ^[5] [CENPL_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.^[6] ^[7] [NKP1_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.^[8] [CENPN_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.^[9]

Publication Abstract from PubMed

In eukaryotes, accurate chromosome segregation in mitosis and meiosis maintains genome stability and prevents aneuploidy. Kinetochores are large protein complexes that, by assembling onto specialized Cenp-A nucleosomes(1,2), function to connect centromeric chromatin to microtubules of the mitotic spindle(3,4). Whereas the centromeres of vertebrate chromosomes comprise millions of DNA base pairs and attach to multiple microtubules, the simple point centromeres of budding yeast are connected to individual microtubules(5,6). All 16 budding yeast chromosomes assemble complete kinetochores using a single Cenp-A nucleosome (Cenp-A(Nuc)), each of which is perfectly centred on its cognate centromere(7-9). The inner and outer kinetochore modules are responsible for interacting with centromeric chromatin and microtubules, respectively. Here we describe the cryo-electron microscopy structure of the Saccharomyces cerevisiae inner kinetochore module, the constitutive centromere associated network (CCAN) complex, assembled onto a Cenp-A nucleosome (CCAN-Cenp-A(Nuc)). The structure explains the interdependency of the constituent subcomplexes of CCAN and shows how the Y-shaped opening of CCAN accommodates Cenp-A(Nuc) to enable specific CCAN subunits to contact the nucleosomal DNA and histone subunits. Interactions with the unwrapped DNA duplex at the two termini of Cenp-A(Nuc) are mediated predominantly by a DNA-binding groove in the Cenp-L-Cenp-N subcomplex. Disruption of these interactions impairs assembly of CCAN onto Cenp-A(Nuc). Our data indicate a mechanism of Cenp-A nucleosome recognition by CCAN and how CCAN acts as a platform for assembly of the outer kinetochore to link centromeres to the mitotic spindle for chromosome segregation.

Structure of the inner kinetochore CCAN complex assembled onto a centromeric nucleosome.,Yan K, Yang J, Zhang Z, McLaughlin SH, Chang L, Fasci D, Ehrenhofer-Murray AE, Heck AJR, Barford D Nature. 2019 Oct 2. pii: 10.1038/s41586-019-1609-1. doi:, 10.1038/s41586-019-1609-1. PMID:31578520^[10]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Poddar A, Roy N, Sinha P. MCM21 and MCM22, two novel genes of the yeast Saccharomyces cerevisiae are required for chromosome transmission. Mol Microbiol. 1999 Jan;31(1):349-60. PMID:9987135
↑ Schleiffer A, Maier M, Litos G, Lampert F, Hornung P, Mechtler K, Westermann S. CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nat Cell Biol. 2012 May 6;14(6):604-13. doi: 10.1038/ncb2493. PMID:22561346 doi:http://dx.doi.org/10.1038/ncb2493
↑ Poddar A, Roy N, Sinha P. MCM21 and MCM22, two novel genes of the yeast Saccharomyces cerevisiae are required for chromosome transmission. Mol Microbiol. 1999 Jan;31(1):349-60. PMID:9987135
↑ Ortiz J, Stemmann O, Rank S, Lechner J. A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore. Genes Dev. 1999 May 1;13(9):1140-55. PMID:10323865
↑ De Wulf P, McAinsh AD, Sorger PK. Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes. Genes Dev. 2003 Dec 1;17(23):2902-21. Epub 2003 Nov 21. PMID:14633972 doi:http://dx.doi.org/10.1101/gad.1144403
↑ Ghosh SK, Poddar A, Hajra S, Sanyal K, Sinha P. The IML3/MCM19 gene of Saccharomyces cerevisiae is required for a kinetochore-related process during chromosome segregation. Mol Genet Genomics. 2001 Apr;265(2):249-57. PMID:11361335
↑ Ghosh SK, Sau S, Lahiri S, Lohia A, Sinha P. The Iml3 protein of the budding yeast is required for the prevention of precocious sister chromatid separation in meiosis I and for sister chromatid disjunction in meiosis II. Curr Genet. 2004 Aug;46(2):82-91. Epub 2004 Jul 6. PMID:15241623 doi:http://dx.doi.org/10.1007/s00294-004-0516-6
↑ Schleiffer A, Maier M, Litos G, Lampert F, Hornung P, Mechtler K, Westermann S. CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nat Cell Biol. 2012 May 6;14(6):604-13. doi: 10.1038/ncb2493. PMID:22561346 doi:http://dx.doi.org/10.1038/ncb2493
↑ Roy N, Poddar A, Lohia A, Sinha P. The mcm17 mutation of yeast shows a size-dependent segregational defect of a mini-chromosome. Curr Genet. 1997 Sep;32(3):182-9. PMID:9339342
↑ Yan K, Yang J, Zhang Z, McLaughlin SH, Chang L, Fasci D, Ehrenhofer-Murray AE, Heck AJR, Barford D. Structure of the inner kinetochore CCAN complex assembled onto a centromeric nucleosome. Nature. 2019 Oct 2. pii: 10.1038/s41586-019-1609-1. doi:, 10.1038/s41586-019-1609-1. PMID:31578520 doi:http://dx.doi.org/10.1038/s41586-019-1609-1

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6qle"

@@ Line 3: / Line 3: @@
 <SX load='6qle' size='340' side='right' viewer='molstar' caption='[[6qle]], [[Resolution|resolution]] 3.55&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6qle]] is a 11 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QLE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6qle]] is a 11 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QLE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6QLE FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FOSTERSB_4937 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), NKP1, YDR383C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), NKP2, YLR315W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), SCRG_04321 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), MCM22, YJR135C, J2122 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), IML3, MCM19, YBR107C, YBR0836 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), CHL4, CTF17, MCM17, YDR254W, YD9320A.04 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), MCM21, CTF5, YDR318W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), CTF19, MCM18, YPL018W, LPB13W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), OKP1, YGR179C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), AME1, ARP100, YBR211C, YBR1458 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qle OCA], [http://pdbe.org/6qle PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qle RCSB], [http://www.ebi.ac.uk/pdbsum/6qle PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qle ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qle OCA], [http://pdbe.org/6qle PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qle RCSB], [http://www.ebi.ac.uk/pdbsum/6qle PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qle ProSAT]</span></td></tr>
 </table>
 == Function ==

6qle

From Proteopedia

Current revision

Structure of inner kinetochore CCAN complex

Structural highlights

Function

Publication Abstract from PubMed

References

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