This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

6r21

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Cryo-EM structure of T7 bacteriophage fiberless tail complex

6r21, resolution 3.33Å

Structural highlights

6r21 is a 30 chain structure with sequence from Bpt7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	6qwp, 6qx5, 6qxm
Gene:	11 (BPT7), 12 (BPT7)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PORTL_BPT7] Forms the portal vertex of the capsid. This portal plays critical roles in head assembly, genome packaging, neck/tail attachment, and genome ejection. The portal protein multimerizes as a single ring-shaped homododecamer arranged around a central channel.[HAMAP-Rule:MF_04120]^[1] ^[2] [TUBE2_BPT7] Structural component of the short non-contractile tail. The tail complex is involved in viral genome delivery. Forms the end of the tail, including the canonical tube, the nozzle, and the small extensions below the fibers. Once the tail tubular structure is formed, the interface between gp11 and gp12 generates the proper environment to interact with the six gp17 trimers. [TUBE1_BPT7] Structural component of the short non-contractile tail. The tail complex is involved in viral genome delivery. Forms the 12-fold symmetric toroidal domain immediately below the connector protein gp8. Once the tail tubular structure is formed, the interface between gp11 and gp12 generates the proper environment to interact with the six fibers, each made of gp17 trimers.

Publication Abstract from PubMed

Double-stranded DNA bacteriophages package their genome at high pressure inside a procapsid through the portal, an oligomeric ring protein located at a unique capsid vertex. Once the DNA has been packaged, the tail components assemble on the portal to render the mature infective virion. The tail tightly seals the ejection conduit until infection, when its interaction with the host membrane triggers the opening of the channel and the viral genome is delivered to the host cell. Using high-resolution cryo-electron microscopy and X-ray crystallography, here we describe various structures of the T7 bacteriophage portal and fiber-less tail complex, which suggest a possible mechanism for DNA retention and ejection: a portal closed conformation temporarily retains the genome before the tail is assembled, whereas an open portal is found in the tail. Moreover, a fold including a seven-bladed beta-propeller domain is described for the nozzle tail protein.

Structures of T7 bacteriophage portal and tail suggest a viral DNA retention and ejection mechanism.,Cuervo A, Fabrega-Ferrer M, Machon C, Conesa JJ, Fernandez FJ, Perez-Luque R, Perez-Ruiz M, Pous J, Vega MC, Carrascosa JL, Coll M Nat Commun. 2019 Aug 20;10(1):3746. doi: 10.1038/s41467-019-11705-9. PMID:31431626^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Agirrezabala X, Martin-Benito J, Valle M, Gonzalez JM, Valencia A, Valpuesta JM, Carrascosa JL. Structure of the connector of bacteriophage T7 at 8A resolution: structural homologies of a basic component of a DNA translocating machinery. J Mol Biol. 2005 Apr 15;347(5):895-902. doi: 10.1016/j.jmb.2005.02.005. PMID:15784250 doi:http://dx.doi.org/10.1016/j.jmb.2005.02.005
↑ Cuervo A, Pulido-Cid M, Chagoyen M, Arranz R, Gonzalez-Garcia VA, Garcia-Doval C, Caston JR, Valpuesta JM, van Raaij MJ, Martin-Benito J, Carrascosa JL. Structural Characterization Of The Bacteriophage T7 Tail Machinery. J Biol Chem. 2013 Jul 24. PMID:23884409 doi:10.1074/jbc.M113.491209
↑ Cuervo A, Fabrega-Ferrer M, Machon C, Conesa JJ, Fernandez FJ, Perez-Luque R, Perez-Ruiz M, Pous J, Vega MC, Carrascosa JL, Coll M. Structures of T7 bacteriophage portal and tail suggest a viral DNA retention and ejection mechanism. Nat Commun. 2019 Aug 20;10(1):3746. doi: 10.1038/s41467-019-11705-9. PMID:31431626 doi:http://dx.doi.org/10.1038/s41467-019-11705-9

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6r21"

@@ Line 3: / Line 3: @@
 <SX load='6r21' size='340' side='right' viewer='molstar' caption='[[6r21]], [[Resolution|resolution]] 3.33&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6r21]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R21 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6r21]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R21 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6R21 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6qwp|6qwp]], [[6qx5|6qx5]], [[6qxm|6qxm]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 BPT7]), 12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 BPT7])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r21 OCA], [http://pdbe.org/6r21 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r21 RCSB], [http://www.ebi.ac.uk/pdbsum/6r21 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r21 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6r21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r21 OCA], [http://pdbe.org/6r21 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r21 RCSB], [http://www.ebi.ac.uk/pdbsum/6r21 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r21 ProSAT]</span></td></tr>
 </table>
 == Function ==

6r21

From Proteopedia

Current revision

Cryo-EM structure of T7 bacteriophage fiberless tail complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox