6s4c

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(Difference between revisions)

Revision as of 07:16, 15 April 2020

Crystal Structure of the vWFA2 subdomain of type VII collagen

Structural highlights

6s4c is a 1 chain structure with sequence from Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Gene:	Col7a1 (LK3 transgenic mice)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CO7A1_MOUSE] Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.[UniProtKB:Q02388]^[1]

Publication Abstract from PubMed

Type VII collagen is an extracellular matrix protein which is important for skin stability; however, detailed information at the molecular level is scarce. The second vWFA (von-Willebrand-factor type A) domain of type VII collagen mediates important interactions, and immunization of mice induces skin blistering in certain strains. To understand vWFA2 function and the pathophysiological mechanisms leading to skin blistering, we structurally characterized this domain by X-ray crystallography and NMR-spectroscopy. Cell adhesion assays identified two new interactions: one with beta1 integrin via its RGD motif and one with laminin-332. The latter interaction was confirmed by surface plasmon resonance with a KD of about 1 mM. These data show that vWFA2 has additional functions in the extracellular matrix besides interacting with type I collagen.

Structural and biophysical characterization of the type VII collagen vWFA2 subdomain leads to identification of two binding sites.,Gebauer JM, Flachsenberg F, Windler C, Richer B, Baumann U, Seeger K FEBS Open Bio. 2020 Feb 7. doi: 10.1002/2211-5463.12807. PMID:32031736^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Heinonen S, Mannikko M, Klement JF, Whitaker-Menezes D, Murphy GF, Uitto J. Targeted inactivation of the type VII collagen gene (Col7a1) in mice results in severe blistering phenotype: a model for recessive dystrophic epidermolysis bullosa. J Cell Sci. 1999 Nov;112 ( Pt 21):3641-8. PMID:10523500
↑ Gebauer JM, Flachsenberg F, Windler C, Richer B, Baumann U, Seeger K. Structural and biophysical characterization of the type VII collagen vWFA2 subdomain leads to identification of two binding sites. FEBS Open Bio. 2020 Feb 7. doi: 10.1002/2211-5463.12807. PMID:32031736 doi:http://dx.doi.org/10.1002/2211-5463.12807

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6s4c"

@@ Line 3: / Line 3: @@
 <StructureSection load='6s4c' size='340' side='right'caption='[[6s4c]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6s4c]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S4C FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6s4c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S4C OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6S4C FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDT:{[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC+ACID'>EDT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDT:{[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC+ACID'>EDT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s4c OCA], [http://pdbe.org/6s4c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s4c RCSB], [http://www.ebi.ac.uk/pdbsum/6s4c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s4c ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Col7a1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6s4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s4c OCA], [http://pdbe.org/6s4c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s4c RCSB], [http://www.ebi.ac.uk/pdbsum/6s4c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s4c ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
+[[Category: Lk3 transgenic mice]]
 [[Category: Baumann, U]]
 [[Category: Flachsenberg, F]]

6s4c

From Proteopedia

Revision as of 07:16, 15 April 2020

Crystal Structure of the vWFA2 subdomain of type VII collagen

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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