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1 bd oxidase; Geobacillus thermodenitrificans
2 Introduction
3 Biological Importance of O₂ reduction
- 3.1 References
- 3.2 Student Contributors

bd oxidase; Geobacillus thermodenitrificans

Introduction

is an integral membrane protein that catalyzes the reduction of oxygen to water using quinol as the reducing substrate.^[1] The full reaction is O₂ + 4H⁺ + 4e^- → 2H₂O. The reaction is electrogenic but it is not coupled to a proton pump. Instead, bd oxidase utilizes internal water molecules to provide the four protons needed for the reduction reaction. and an external ubiquinone molecule for the four electrons needed.^[2] bd oxidase plays a key role in protecting the organism from high oxidative stress. In a gram-negative bacteria heterotrophs like Geobacillus thermodenitrificans, bd oxidase prevents free radicals in the intracellular space. Other organisms, like humans, have mechanisms that do the same thing but are more intricate due to the organism’s higher levels of complexity.

There are two main types of respiratory cytochrome oxidases: the heme/copper oxidases and the heme-only cytochrome bd quinol oxidase, which is what bd oxidase falls under.^[3] Heme-only cytochrome bd quinol oxidases are associated with microaerobic dioxygen respiration, and they have a high affinity for oxygen.

The Geobacillus thermodenitrificans is a facultative aerobic thermophilic bacterium that utilizes the bd oxidase mechanism. The oxygen enters the enzyme through the selective that funnels the extracellular oxygen to in the active site. The electrons for the reaction are provided by ubiquinone molecule bound to the . The protons for the reaction are provided by one of two , either the or . Both of the proton pathways utilize the intracellular water molecules for the proton source, and shuttle them to .

1 Structure
2 Overall Oxygen Reduction Mechanism Summary
3 Structure Similarity to bd oxidase found in E. coli

Structure

The focus of this page is to explain the structure and function of the G. thermodenitrificans’ bd oxidase. The contains that are arranged in a nearly oval shape.^[2] The protein contains two structurally similar subunits, , seen in blue, and , seen in red, each containing nine helices, and one smaller subunit, , in teal, with one transmembrane helix. These subunits interact using hydrophobic residues and symmetry at the interfaces. The CydX subunit, whose function is not currently known, is positioned in the same way as CydS, a separate subunit that is found in the bd oxidase homologue from E. coli bd oxidase, but is not found in G. thermodenitrificans. Due to its similar structure and position to CydS, CydX has been hypothesized to potentially stabilize during potential structural rearrangements of the Q loop upon binding and oxidation of ubiquinone (Fig. 1), the function of CydS in E. coli.^[2] The is a hydrophilic region above Cyd A. The lack of hydrogen bonding in this hydrophobic protein allows the protein to be flexible and go through a large conformational change for reduction of dioxygen. is mostly involved in the proton pathway, and is involved with the oxygen pathway.

Other structures of bd oxidase exist that contain a variety of potential routes for the different reactants of the reduction of oxygen. For example, the bd oxidase of E. coli contains a different orientation of the Hemes and many different mechanisms of proton shuttling. G. thermodenitrificans was chosen because of the interest in the unique proton pathways, as described in the “Potential Proton Pathways” section.

Active Site

Figure 1. The active site of bd oxidase for G. thermodenitrificans. Heme B558 (pink; left), Heme B595 (pink; right), and Heme D (green). Important residues shown in blue. Measurements are shown in Å.

The active site for bd oxidase in G. thermodenitrificans is located in subunit Cyd A. The site consists of three iron hemes: , , and that are held together in a rigid triangular due to Van der Waals interactions.^[2] The between each heme's central iron is relatively constant which serves to shuttle protons and electrons from one heme to another efficiently (Fig. 1). is hypothesized to act as an electron acceptor, orientated toward the extracellular side by (Fig. 1).^[2] With closest in proximity to the intracellular side, Heme B559 is likely the proton acceptor with two potential proton pathways. Both and then shuttle their respective ions directly to as this is the shortest pathway.

Potential Oxygen Entry Site

is the hypothesized spot for the to enter the protein. Heme D (shown in green) is directly connected to the protein surface on CydA and contains a solvent accessible substrate channel. This channel and accessibility allow for oxygen to easily bind to Heme D and eventually be reduced to two H₂O molecules. This process requires a proton and electron source, both described in the later sections.

Electron Source

An electron source is needed in order for the redox reaction of O₂ to occur. Cytochrome bd oxidase uses the quinol molecule ubiquinone as an electron donor (Figure 2).

Figure 2. Chemical structure of ubiquinone.

As shown in the , the is on the extracellular surface and provides a binding site for ubiquinone.^[2] Heme and thus is the suggested electron acceptor. This suggestion is further supported by the often found as intermediate electron receptors in biological electron transfer chains.^[2]

Potential Proton Pathways

Figure 3. Two potential sources of protons: CydA and CydB pathway.

Because there is no proton pump present, the proton transfer mechanism is facilitated by via intracellular water molecules.

One potential proton pathway is formed from the of . It is called the . The residues along this pathway help facilitate the movement of the protons. The location of in the structure is a key residue in this pathway. Its location within the pathway and negative charge characteristic implies that this glutamate residue is a redox state-dependent mediator of proton transfer. In other words, it acts like a proton shuttle.^[2] The , which is the last residue in this pathway, could be the protonatable group eventually used upon reduction. More research needs to be done to determine whether the CydA pathway is solely providing protons for charge compensation, or whether can be a branching point that is able to pass protons via the propionates to the oxygen-binding site.^[4]

Another potential entry site is related to the of . Therefore, this is called the . In this pathway, is thought to be the equivalent of the in the CydA pathway.^[2] The other residues help facilitate the movement of the proton very similarly to the CydA pathway. There is less known about the , and therefore, the is the most accepted source of protons.

Overall Oxygen Reduction Mechanism Summary

Figure 4. Overall oxidation-reduction mechanism summary.

As mentioned above, the purpose of the bd oxidase is to reduce O₂ to 2H₂O using quinol as the reducing substrate, and having the overall reaction of O₂ + 4H⁺ + 4e^- → 2H₂O. The oxygen comes from the extracellular side of the protein, and enters through the oxygen entry site to . This pathway is depicted in orange in Figure 4.

The protons that are required in the pathway are not provided by a pump, but rather via intracellular water. The utilize amino acids with charges that help shuttle the protons from the intracellular side of the protein to . in the active site. The passes through the , and is shown in purple in Figure 4. The proceeds through the , and is shown in green in Figure 4.

As shown above, the electrons required for the reduction mechanism come from a ubiquinol molecule (Fig. 2) that simultaneously binds to the and gets oxidized giving 4e^- to . Once at the 4e^- will be shuttled directly to to be used in the reduction of O₂. The electron pathway is depicted in blue in Figure 4.

When all of these elements of the reduction aggregate in the active site, the protons and electrons are shuttled to , where the actual reduction occurs. The 2H₂O molecules are then expelled, as seen in red in Figure 4. The shuttling of these electrons and protons also helps assist with the electric chemical potential in the cellular membrane.

Structure Similarity to bd oxidase found in E. coli

Figure 5. Alignment of bd oxidase for the organisms G. thermodenitrificans (PDB: 5doq) shown in blue and E. coli (PDB: 6rko) shown in purple.

Figure 6. Heme arrangements for the organisms G. thermodenitrificans and E. coli. Heme D (green); Heme B595 and Heme B558 shown in pink

The structure of bd oxidase for G. thermodenitrificans is highly similar to the structure of bd oxidase in E. coli with the major differentiation being the length of the Q-loop.^[5] The similarities and differences between the two proteins can be seen in the alignment of their main structures (Fig.5). Although only having one significant difference in structure, this shift in the X-loop causes the two proteins to have different active sites (Fig. 6). In particular, the are arranged differently than the . The main reason for this change in heme arrangement is because of the being located differently in E. coli, thus causing a different active site arrangement in the protein.^[2]

Biological Importance of O₂ reduction

Oxygen toxicity is a fatal problem among all organisms, but can easily occur in prokaryotes due to their low oxygen tolerance. In prokaryotes, the cytochrome bd oxygen reductases function to quickly reduce the concentration of O₂ into H₂O to protect the cell from detrimental effects. Without proper functioning of these enzymes, or if O₂ concentrations are too high, the concentrations of the intermediates formed from the reduction reaction will increase and can be detrimental. As a result of the vitality of reducing O₂ in prokaryotes, knowledge on bd oxidases can help develop drugs that target these enzymes to combat bacterial infection.^[6]

References

↑ Giuffre A, Borisov VB, Arese M, Sarti P, Forte E. Cytochrome bd oxidase and bacterial tolerance to oxidative and nitrosative stress. Biochim Biophys Acta. 2014 Jul;1837(7):1178-87. doi:, 10.1016/j.bbabio.2014.01.016. Epub 2014 Jan 31. PMID:24486503 doi:http://dx.doi.org/10.1016/j.bbabio.2014.01.016
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 ^2.9 Safarian S, Hahn A, Mills DJ, Radloff M, Eisinger ML, Nikolaev A, Meier-Credo J, Melin F, Miyoshi H, Gennis RB, Sakamoto J, Langer JD, Hellwig P, Kuhlbrandt W, Michel H. Active site rearrangement and structural divergence in prokaryotic respiratory oxidases. Science. 2019 Oct 4;366(6461):100-104. doi: 10.1126/science.aay0967. PMID:31604309 doi:http://dx.doi.org/10.1126/science.aay0967
↑ Das A, Silaghi-Dumitrescu R, Ljungdahl LG, Kurtz DM Jr. Cytochrome bd oxidase, oxidative stress, and dioxygen tolerance of the strictly anaerobic bacterium Moorella thermoacetica. J Bacteriol. 2005 Mar;187(6):2020-9. doi: 10.1128/JB.187.6.2020-2029.2005. PMID:15743950 doi:http://dx.doi.org/10.1128/JB.187.6.2020-2029.2005
↑ Safarian S, Rajendran C, Muller H, Preu J, Langer JD, Ovchinnikov S, Hirose T, Kusumoto T, Sakamoto J, Michel H. Structure of a bd oxidase indicates similar mechanisms for membrane-integrated oxygen reductases. Science. 2016 Apr 29;352(6285):583-6. doi: 10.1126/science.aaf2477. PMID:27126043 doi:http://dx.doi.org/10.1126/science.aaf2477
↑ Thesseling A, Rasmussen T, Burschel S, Wohlwend D, Kagi J, Muller R, Bottcher B, Friedrich T. Homologous bd oxidases share the same architecture but differ in mechanism. Nat Commun. 2019 Nov 13;10(1):5138. doi: 10.1038/s41467-019-13122-4. PMID:31723136 doi:http://dx.doi.org/10.1038/s41467-019-13122-4
↑ Borisov VB, Gennis RB, Hemp J, Verkhovsky MI. The cytochrome bd respiratory oxygen reductases. Biochim Biophys Acta. 2011 Nov;1807(11):1398-413. doi:, 10.1016/j.bbabio.2011.06.016. Epub 2011 Jul 1. PMID:21756872 doi:http://dx.doi.org/10.1016/j.bbabio.2011.06.016

Student Contributors

Emma H Harris

Carson E Middlebrook

Retrieved from "http://52.214.119.220/wiki/index.php/Sandbox_Reserved_1600"

@@ Line 10: / Line 10: @@
 =Structure=
-The focus of this page is to explain the structure and function of the [https://www.rcsb.org/structure/5DOQ ''Geobacillus thermodenitrificans’'' bd oxidase]. The <scene name='83/838655/Bdoxidase_structure_full/4'>overall structure</scene> contains <scene name='83/838655/Bdoxidase_only_helicies/2'> 19 transmembrane helices</scene> that are arranged in a nearly oval shape.<ref name = ”Safarian” /> The protein contains two structurally similar subunits, <scene name='83/838655/Bdoxidase_cyda_subunit/2'>CydA</scene>, seen in <font color='blue'><b>blue</b></font>, and <scene name='83/838655/Bdoxidase_cydb_subunit/2'>CydB</scene>, seen in <font color='red'><b>red</b></font>, each containing nine helices, and one smaller subunit, <scene name='83/838655/Bdoxidase_cydx_subunit/2'>CydX</scene>, in <font color='teal'><b>teal</b></font>, with one transmembrane helix. These subunits interact using hydrophobic residues and symmetry at the interfaces. The CydX subunit, whose function is not currently known, is positioned in the same way as CydS, a separate subunit that is found in the bd oxidase homologue from [https://www.rcsb.org/structure/6RKO ''E. coli'' bd oxidase] but is not found in ''G. thermodenitrificans''. Due to its similar structure and position to CydS, CydX has been hypothesized to potentially stabilize <scene name='83/838655/Bd_oxidase_heme_558/3'>Heme B558</scene> during potential structural rearrangements of the Q loop upon binding and oxidation of ubiquinone (Fig. 1), the function of CydS in ''E. coli.''<ref name = ”Safarian” /> The <scene name='83/838655/Bdoxidase_q_loop/2'>Q loop</scene> is a hydrophilic region above Cyd A. The lack of [https://en.wikipedia.org/wiki/Hydrogen_bond hydrogen bonding] in this hydrophobic protein allows the protein to be flexible and go through a large conformational change for reduction of dioxygen. <scene name='83/838655/Bd_oxidase_heme_b_595/2'>Heme B595</scene> is mostly involved in the proton pathway, and <scene name='83/838655/Bd_oxidase_heme_d/2'>Heme D</scene> is involved with the oxygen pathway.
+The focus of this page is to explain the structure and function of the [https://www.rcsb.org/structure/5DOQ ''G. thermodenitrificans’'' bd oxidase]. The <scene name='83/838655/Bdoxidase_structure_full/4'>overall structure</scene> contains <scene name='83/838655/Bdoxidase_only_helicies/2'> 19 transmembrane helices</scene> that are arranged in a nearly oval shape.<ref name = ”Safarian” /> The protein contains two structurally similar subunits, <scene name='83/838655/Bdoxidase_cyda_subunit/2'>CydA</scene>, seen in <font color='blue'><b>blue</b></font>, and <scene name='83/838655/Bdoxidase_cydb_subunit/2'>CydB</scene>, seen in <font color='red'><b>red</b></font>, each containing nine helices, and one smaller subunit, <scene name='83/838655/Bdoxidase_cydx_subunit/2'>CydX</scene>, in <font color='teal'><b>teal</b></font>, with one transmembrane helix. These subunits interact using hydrophobic residues and symmetry at the interfaces. The CydX subunit, whose function is not currently known, is positioned in the same way as CydS, a separate subunit that is found in the bd oxidase homologue from [https://www.rcsb.org/structure/6RKO ''E. coli'' bd oxidase], but is not found in ''G. thermodenitrificans''. Due to its similar structure and position to CydS, CydX has been hypothesized to potentially stabilize <scene name='83/838655/Bd_oxidase_heme_558/3'>Heme B558</scene> during potential structural rearrangements of the Q loop upon binding and oxidation of ubiquinone (Fig. 1), the function of CydS in ''E. coli.''<ref name = ”Safarian” /> The <scene name='83/838655/Bdoxidase_q_loop/2'>Q loop</scene> is a hydrophilic region above Cyd A. The lack of [https://en.wikipedia.org/wiki/Hydrogen_bond hydrogen bonding] in this hydrophobic protein allows the protein to be flexible and go through a large conformational change for reduction of dioxygen. <scene name='83/838655/Bd_oxidase_heme_b_595/2'>Heme B595</scene> is mostly involved in the proton pathway, and <scene name='83/838655/Bd_oxidase_heme_d/2'>Heme D</scene> is involved with the oxygen pathway.
-Other structures of bd oxidase exist that contain a variety of potential routes for the different reactants of the reduction of oxygen. For example, the bd oxidase of ''E. coli'' contains a different orientation of the Hemes and many different mechanisms of proton shuttling. ''Geobacillus thermodenitrificans'' was chosen because of the interest in the unique proton pathways, as described in the “Potential Proton Pathways” section.
+Other structures of bd oxidase exist that contain a variety of potential routes for the different reactants of the reduction of oxygen. For example, the bd oxidase of ''E. coli'' contains a different orientation of the Hemes and many different mechanisms of proton shuttling. ''G. thermodenitrificans'' was chosen because of the interest in the unique proton pathways, as described in the “Potential Proton Pathways” section.
 ==Active Site==
-[[Image:Hemes2.png|300 px|right|thumb|Figure 1. The active site of bd oxidase for ''Geobacillus thermodenitrificans''. Heme B558 (pink; left), Heme B595 (pink; right), and Heme D (green). Important residues shown in blue. Measurements are shown in Å.]]
+[[Image:Hemes2.png|300 px|right|thumb|Figure 1. The active site of bd oxidase for ''G. thermodenitrificans''. Heme B558 (pink; left), Heme B595 (pink; right), and Heme D (green). Important residues shown in blue. Measurements are shown in Å.]]
 The active site for bd oxidase in ''G. thermodenitrificans'' is located in subunit Cyd A. The site consists of three iron hemes: <scene name='83/838655/Bd_oxidase_heme_558/3'>Heme B558</scene>, <scene name='83/838655/Bd_oxidase_heme_b_595/2'>Heme B595</scene>, and <scene name='83/838655/Bd_oxidase_heme_d/2'>Heme D</scene> that are held together in a rigid triangular <scene name='83/838655/Hemes/8'>arrangement</scene> due to [https://en.wikipedia.org/wiki/Van_der_Waals_force Van der Waals interactions].<ref name = ”Safarian” /> The <scene name='83/838655/Hemes_measurements/5'>length</scene> between each heme's central iron is relatively constant which serves to shuttle protons and electrons from one heme to another efficiently (Fig. 1). <scene name='83/838655/Bd_oxidase_heme_558/3'>Heme B558</scene>  is hypothesized to act as an electron acceptor, orientated toward the extracellular side by <scene name='83/838655/Bdoxidase_structure_heme/4'>His 186, Met 325, and Lys 252</scene> (Fig. 1).<ref name = ”Safarian” /> With <scene name='83/838655/Bd_oxidase_heme_b_595/2'>Heme B595</scene> closest in proximity to the intracellular side, Heme B559 is likely the proton acceptor with two potential proton pathways. Both <scene name='83/838655/Bd_oxidase_heme_558/3'>Heme B558</scene> and <scene name='83/838655/Bd_oxidase_heme_b_595/2'>Heme B595</scene> then shuttle their respective ions directly to <scene name='83/838655/Bd_oxidase_heme_d/2'>Heme D</scene> as this is the shortest pathway.