5eah

From Proteopedia

(Difference between revisions)

Revision as of 06:50, 22 April 2020

Saccharomyces cerevisiae CYP51 complexed with the plant pathogen inhibitor Difenoconazole

Structural highlights

5eah is a 1 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , , ,
Related:	4wmz, 5eab, 5eac, 5ead, 5eae, 5eaf, 5eag
Gene:	ERG11, SCY_2394 (Baker's yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Azole antifungals, known as demethylase inhibitors (DMIs), target sterol 14alpha-demethylase (CYP51) in the ergosterol biosynthetic pathway of fungal pathogens of both plants and humans. DMIs remain the treatment of choice in crop protection against a wide range of fungal phytopathogens that have the potential to reduce crop yields and threaten food security. We used a yeast membrane protein expression system to overexpress recombinant hexahistidine-tagged S. cerevisiae lanosterol 14alpha-demethylase and the Y140F or Y140H mutants of this enzyme as surrogates in order characterize interactions with DMIs. The whole-cell antifungal activity (MIC50 values) of both the R- and S-enantiomers of tebuconazole, prothioconazole (PTZ), prothioconazole-desthio, and oxo-prothioconazole (oxo-PTZ) as well as for fluquinconazole, prochloraz and a racemic mixture of difenoconazole were determined. In vitro binding studies with the affinity purified enzyme were used to show tight type II binding to the yeast enzyme for all compounds tested except PTZ and oxo-PTZ. High resolution X-ray crystal structures of ScErg11p6xHis in complex with seven DMIs, including four enantiomers, reveal triazole-mediated coordination of all compounds and the specific orientation of compounds within the relatively hydrophobic binding site. Comparison with CYP51 structures from fungal pathogens including Candida albicans, Candida glabrata and Aspergillus fumigatus provides strong evidence for a highly conserved CYP51 structure including the drug binding site. The structures obtained using S. cerevisiae lanosterol 14alpha-demethylase in complex with these agrochemicals provide the basis for understanding the impact of mutations on azole susceptibility and a platform for the structure-directed design of the next-generation of DMIs.

Structural and Functional Elucidation of Yeast Lanosterol 14alpha-Demethylase in Complex with Agrochemical Antifungals.,Tyndall JD, Sabherwal M, Sagatova AA, Keniya MV, Negroni J, Wilson RK, Woods MA, Tietjen K, Monk BC PLoS One. 2016 Dec 1;11(12):e0167485. doi: 10.1371/journal.pone.0167485., eCollection 2016. PMID:27907120^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Tyndall JD, Sabherwal M, Sagatova AA, Keniya MV, Negroni J, Wilson RK, Woods MA, Tietjen K, Monk BC. Structural and Functional Elucidation of Yeast Lanosterol 14alpha-Demethylase in Complex with Agrochemical Antifungals. PLoS One. 2016 Dec 1;11(12):e0167485. doi: 10.1371/journal.pone.0167485., eCollection 2016. PMID:27907120 doi:http://dx.doi.org/10.1371/journal.pone.0167485

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5eah"

@@ Line 1: / Line 1: @@
 ==Saccharomyces cerevisiae CYP51 complexed with the plant pathogen inhibitor Difenoconazole==
-<StructureSection load='5eah' size='340' side='right' caption='[[5eah]], [[Resolution|resolution]] 2.54&Aring;' scene=''>
+<StructureSection load='5eah' size='340' side='right'caption='[[5eah]], [[Resolution|resolution]] 2.54&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5eah]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EAH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EAH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5eah]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EAH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EAH FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5LW:1-[[(2~{S},4~{S})-2-[2-CHLORANYL-4-(4-CHLORANYLPHENOXY)PHENYL]-4-METHYL-1,3-DIOXOLAN-2-YL]METHYL]-1,2,4-TRIAZOLE'>5LW</scene>, <scene name='pdbligand=5LX:1-[[(2~{R},4~{S})-2-[2-CHLORANYL-4-(4-CHLORANYLPHENOXY)PHENYL]-4-METHYL-1,3-DIOXOLAN-2-YL]METHYL]-1,2,4-TRIAZOLE'>5LX</scene>, <scene name='pdbligand=5LY:1-[[(2~{R},4~{R})-2-[2-CHLORANYL-4-(4-CHLORANYLPHENOXY)PHENYL]-4-METHYL-1,3-DIOXOLAN-2-YL]METHYL]-1,2,4-TRIAZOLE'>5LY</scene>, <scene name='pdbligand=5LZ:1-[[(2~{S},4~{R})-2-[2-CHLORANYL-4-(4-CHLORANYLPHENOXY)PHENYL]-4-METHYL-1,3-DIOXOLAN-2-YL]METHYL]-1,2,4-TRIAZOLE'>5LZ</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5LW:1-[[(2~{S},4~{S})-2-[2-CHLORANYL-4-(4-CHLORANYLPHENOXY)PHENYL]-4-METHYL-1,3-DIOXOLAN-2-YL]METHYL]-1,2,4-TRIAZOLE'>5LW</scene>, <scene name='pdbligand=5LX:1-[[(2~{R},4~{S})-2-[2-CHLORANYL-4-(4-CHLORANYLPHENOXY)PHENYL]-4-METHYL-1,3-DIOXOLAN-2-YL]METHYL]-1,2,4-TRIAZOLE'>5LX</scene>, <scene name='pdbligand=5LY:1-[[(2~{R},4~{R})-2-[2-CHLORANYL-4-(4-CHLORANYLPHENOXY)PHENYL]-4-METHYL-1,3-DIOXOLAN-2-YL]METHYL]-1,2,4-TRIAZOLE'>5LY</scene>, <scene name='pdbligand=5LZ:1-[[(2~{S},4~{R})-2-[2-CHLORANYL-4-(4-CHLORANYLPHENOXY)PHENYL]-4-METHYL-1,3-DIOXOLAN-2-YL]METHYL]-1,2,4-TRIAZOLE'>5LZ</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4wmz|4wmz]], [[5eab|5eab]], [[5eac|5eac]], [[5ead|5ead]], [[5eae|5eae]], [[5eaf|5eaf]], [[5eag|5eag]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5eah FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eah OCA], [http://pdbe.org/5eah PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eah RCSB], [http://www.ebi.ac.uk/pdbsum/5eah PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eah ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ERG11, SCY_2394 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=307796 Baker's yeast])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5eah FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eah OCA], [http://pdbe.org/5eah PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eah RCSB], [http://www.ebi.ac.uk/pdbsum/5eah PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eah ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Baker's yeast]]
+[[Category: Large Structures]]
 [[Category: Keniya, M V]]
 [[Category: Monk, B C]]

5eah

From Proteopedia

Revision as of 06:50, 22 April 2020

Saccharomyces cerevisiae CYP51 complexed with the plant pathogen inhibitor Difenoconazole

Structural highlights

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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