Sandbox Reserved 897
From Proteopedia
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== Overview == | == Overview == | ||
| - | The PWWP domain belongs to the Royal family, which consists of Tudor, chromodomain, MBT(Malignant Brain Tumor), and PWWP domains. It was first identified as a structural motif includes 100 to 130 amino acids. The name PWWP comes after its very conservative sequence, Pro-Trp-Trp-Pro located at its β2 region.<ref>Rona GB, Eleutherio EC, Pinheiro AS. PWWP domains and their modes of sensing DNA and histone methylated lysines. Biophysical reviews. 2016 Mar 1;8(1):63-74.</ref> This domain is mainly related to the recognition of methylated histone tail lysine and related to the epigenetic regulation of genes. | + | The PWWP domain belongs to the Royal family, which consists of Tudor, chromodomain, MBT(Malignant Brain Tumor), and PWWP domains. It was first identified as a structural motif includes 100 to 130 amino acids in WHSC1 protein. The name PWWP comes after its very conservative sequence, Pro-Trp-Trp-Pro located at its β2 region.<ref>Rona GB, Eleutherio EC, Pinheiro AS. PWWP domains and their modes of sensing DNA and histone methylated lysines. Biophysical reviews. 2016 Mar 1;8(1):63-74.</ref> This domain is mainly related to the recognition of methylated histone tail lysine and related to the epigenetic regulation of genes. |
== Function == | == Function == | ||
The PWWP domain is also used for protein-protein interaction. This domain also may have nonspecific interaction with a DNA strand, using its abundant lysine and arginine residues. | The PWWP domain is also used for protein-protein interaction. This domain also may have nonspecific interaction with a DNA strand, using its abundant lysine and arginine residues. | ||
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== Structure == | == Structure == | ||
| - | The first proline affects the stability and aggregation of the protein. | + | Two disticntive The first proline affects the stability and aggregation of the protein. The proline gives more stability and oligomerization to the protein, compared to the alanine at the same position.<ref>Hung YL, Lee HJ, Jiang I, Lin SC, Lo WC, Lin YJ, Sue SC. The first residue of the PWWP motif modulates HATH domain binding, Stability, and Protein–Protein Interaction. Biochemistry. 2015 Jul 7;54(26):4063-74.</ref> |
=== Evolutionary conservations === | === Evolutionary conservations === | ||
| - | The loyal family has a common structural characteristic, three conserved β-strands.<ref>Rona GB, Eleutherio EC, Pinheiro AS. PWWP domains and their modes of sensing DNA and histone methylated lysines. Biophysical reviews. 2016 Mar 1;8(1):63-74.</ref> | + | PWWP domain is only found in eukaryotes, from a yeast to a human. The number of PWWP containing protein is also varying on species; the human has 20. The loyal family has a common structural characteristic, three conserved β-strands.<ref>Rona GB, Eleutherio EC, Pinheiro AS. PWWP domains and their modes of sensing DNA and histone methylated lysines. Biophysical reviews. 2016 Mar 1;8(1):63-74.</ref> The most conservative section is the PWWP sequence, which it is named after. |
== Therapheutic features == | == Therapheutic features == | ||
Revision as of 04:16, 27 April 2020
| This Sandbox is Reserved from Jan 13 through July 31, 2020 for use in the course Protein Structure in Drug Discovery taught by Karen C. Glass at the ACPHS, Colchester, United States. This reservation includes Sandbox Reserved 895 through Sandbox Reserved 901. |
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BRPF3 PWWP binding domain 3PFS
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References
- ↑ Rona GB, Eleutherio EC, Pinheiro AS. PWWP domains and their modes of sensing DNA and histone methylated lysines. Biophysical reviews. 2016 Mar 1;8(1):63-74.
- ↑ Hung YL, Lee HJ, Jiang I, Lin SC, Lo WC, Lin YJ, Sue SC. The first residue of the PWWP motif modulates HATH domain binding, Stability, and Protein–Protein Interaction. Biochemistry. 2015 Jul 7;54(26):4063-74.
- ↑ Rona GB, Eleutherio EC, Pinheiro AS. PWWP domains and their modes of sensing DNA and histone methylated lysines. Biophysical reviews. 2016 Mar 1;8(1):63-74.
