1b69

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[[Image:1b69.gif|left|200px]]
[[Image:1b69.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1b69 |SIZE=350|CAPTION= <scene name='initialview01'>1b69</scene>
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The line below this paragraph, containing "STRUCTURE_1b69", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1b69| PDB=1b69 | SCENE= }}
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|RELATEDENTRY=[[1tn9|1TN9]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b69 OCA], [http://www.ebi.ac.uk/pdbsum/1b69 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1b69 RCSB]</span>
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}}
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'''THE SOLUTION STRUCTURE OF TN916 INTEGRASE N-TERMINAL DOMAIN/DNA COMPLEX'''
'''THE SOLUTION STRUCTURE OF TN916 INTEGRASE N-TERMINAL DOMAIN/DNA COMPLEX'''
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[[Category: Connolly, K M.]]
[[Category: Connolly, K M.]]
[[Category: Wojciak, J M.]]
[[Category: Wojciak, J M.]]
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[[Category: beta-sheet recognition]]
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[[Category: Beta-sheet recognition]]
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[[Category: complex]]
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[[Category: Complex]]
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[[Category: dna binding]]
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[[Category: Dna binding]]
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[[Category: integrase]]
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[[Category: Integrase]]
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[[Category: transposition]]
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[[Category: Transposition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:07:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:54:31 2008''
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Revision as of 08:07, 2 May 2008

Image:1b69.gif

Template:STRUCTURE 1b69

THE SOLUTION STRUCTURE OF TN916 INTEGRASE N-TERMINAL DOMAIN/DNA COMPLEX


Overview

The integrase protein catalyzes the excision and integration of the Tn916 conjugative transposon, a promiscuous genetic element that spreads antibiotic resistance in pathogenic bacteria. The solution structure of the N-terminal domain of the Tn916 integrase protein bound to its DNA-binding site within the transposon arm has been determined. The structure reveals an interesting mode of DNA recognition, in which the face of a three-stranded antiparallel beta-sheet is positioned within the major groove. A comparison to the structure of the homing endonuclease I-Ppol-DNA complex suggests that the three-stranded sheet may represent a new DNA-binding motif whose residue composition and position within the major groove are varied to alter specificity. The structure also provides insights into the mechanism of conjugative transposition. The DNA in the complex is bent approximately 35 degrees and may, together with potential interactions between bound integrase proteins at directly repeated sites, significantly bend the arms of the transposon.

About this Structure

1B69 is a Single protein structure of sequence from Enterococcus faecalis. Full crystallographic information is available from OCA.

Reference

NMR structure of the Tn916 integrase-DNA complex., Wojciak JM, Connolly KM, Clubb RT, Nat Struct Biol. 1999 Apr;6(4):366-73. PMID:10201406 Page seeded by OCA on Fri May 2 11:07:26 2008

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