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5oeg

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Revision as of 06:55, 29 April 2020

Molecular tweezers modulate 14-3-3 protein-protein interactions

Structural highlights

5oeg is a 1 chain structure with sequence from Human. This structure supersedes the now removed PDB entry 4hru. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	SFN, HME1 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[1433S_HUMAN] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.

Publication Abstract from PubMed

Supramolecular chemistry has recently emerged as a promising way to modulate protein functions, but devising molecules that will interact with a protein in the desired manner is difficult as many competing interactions exist in a biological environment (with solvents, salts or different sites for the target biomolecule). We now show that lysine-specific molecular tweezers bind to a 14-3-3 adapter protein and modulate its interaction with partner proteins. The tweezers inhibit binding between the 14-3-3 protein and two partner proteins-a phosphorylated (C-Raf) protein and an unphosphorylated one (ExoS)-in a concentration-dependent manner. Protein crystallography shows that this effect arises from the binding of the tweezers to a single surface-exposed lysine (Lys214) of the 14-3-3 protein in the proximity of its central channel, which normally binds the partner proteins. A combination of structural analysis and computer simulations provides rules for the tweezers' binding preferences, thus allowing us to predict their influence on this type of protein-protein interactions.

Molecular tweezers modulate 14-3-3 protein-protein interactions.,Bier D, Rose R, Bravo-Rodriguez K, Bartel M, Ramirez-Anguita JM, Dutt S, Wilch C, Klarner FG, Sanchez-Garcia E, Schrader T, Ottmann C Nat Chem. 2013 Mar;5(3):234-9. doi: 10.1038/nchem.1570. Epub 2013 Feb 17. PMID:23422566^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bier D, Rose R, Bravo-Rodriguez K, Bartel M, Ramirez-Anguita JM, Dutt S, Wilch C, Klarner FG, Sanchez-Garcia E, Schrader T, Ottmann C. Molecular tweezers modulate 14-3-3 protein-protein interactions. Nat Chem. 2013 Mar;5(3):234-9. doi: 10.1038/nchem.1570. Epub 2013 Feb 17. PMID:23422566 doi:http://dx.doi.org/10.1038/nchem.1570

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5oeg"

@@ Line 1: / Line 1: @@
 ==Molecular tweezers modulate 14-3-3 protein-protein interactions==
-<StructureSection load='5oeg' size='340' side='right' caption='[[5oeg]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
+<StructureSection load='5oeg' size='340' side='right'caption='[[5oeg]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5oeg]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4hru 4hru]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OEG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OEG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5oeg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4hru 4hru]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OEG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5OEG FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=9SZ:C42+H32+O8+P2'>9SZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9SZ:C42+H32+O8+P2'>9SZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oeg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oeg OCA], [http://pdbe.org/5oeg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oeg RCSB], [http://www.ebi.ac.uk/pdbsum/5oeg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oeg ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SFN, HME1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5oeg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oeg OCA], [http://pdbe.org/5oeg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oeg RCSB], [http://www.ebi.ac.uk/pdbsum/5oeg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oeg ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 5oeg" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[14-3-3 protein 3D structures|14-3-3 protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Bier, D]]
 [[Category: Ottmann, C]]

5oeg

From Proteopedia

Revision as of 06:55, 29 April 2020

Molecular tweezers modulate 14-3-3 protein-protein interactions

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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