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2ks9

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Revision as of 07:35, 29 April 2020

Solution conformation of substance P in water complexed with NK1R

Structural highlights

2ks9 is a 2 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2ksa, 2ksb
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NK1R_HUMAN] This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K. [TKN1_HUMAN] Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.

Publication Abstract from PubMed

Substance P (SP) is one of the target neurotransmitters associated with diseases related to chronic inflammation, pain and depression. The selective receptor for SP, NK(1)R is located in the heterogeneous microdomains or caveolae in membrane. Gangliosides, specifically GM1, are markers of these heterogeneous sites. Also, gangliosides are considered as important regulatory elements in cell-cell recognition and cell signaling. In the present work, we describe the conformations of Substance P in the presence of ternary membrane systems containing GM1 at the physiological concentration. SP is mostly unstructured in water, but appears as extended 3(10) helical or turn III in isotropic bicelles, more pronounced in the presence of GM1. NMR results suggest that, in the GM1 containing bicelles, the peptide is more inserted into the membrane with its C-terminus, while N-terminus lies close to the membrane-water interface. The NMR-derived conformation of SP in GM1 bicelles is docked on homology modeled NK(1)R and resulting interactions satisfy reported mutagenesis, fluorescence, photo-affinity labeling and modeling data. The results highlight efficacy of GM1 in membrane in providing structure in an otherwise flexible neurotransmitter Substance P; thus providing indication that it may be useful also for other neurotransmitter peptides/proteins associated with membrane.

NMR evidence of GM1-induced conformational change of Substance P using isotropic bicelles.,Gayen A, Goswami SK, Mukhopadhyay C Biochim Biophys Acta. 2010 Oct 16. PMID:20937248^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gayen A, Goswami SK, Mukhopadhyay C. NMR evidence of GM1-induced conformational change of Substance P using isotropic bicelles. Biochim Biophys Acta. 2010 Oct 16. PMID:20937248 doi:10.1016/j.bbamem.2010.09.023

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ks9"

@@ Line 1: / Line 1: @@
 ==Solution conformation of substance P in water complexed with NK1R==
-<StructureSection load='2ks9' size='340' side='right' caption='[[2ks9]], [[NMR_Ensembles_of_Models | 5 NMR models]]' scene=''>
+<StructureSection load='2ks9' size='340' side='right'caption='[[2ks9]], [[NMR_Ensembles_of_Models | 5 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2ks9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KS9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2ks9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2KS9 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ksa|2ksa]], [[2ksb|2ksb]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ks9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ks9 OCA], [http://pdbe.org/2ks9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ks9 RCSB], [http://www.ebi.ac.uk/pdbsum/2ks9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ks9 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2ks9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ks9 OCA], [http://pdbe.org/2ks9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ks9 RCSB], [http://www.ebi.ac.uk/pdbsum/2ks9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ks9 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 23: @@
 </StructureSection>
 [[Category: Homo sapiens]]
+[[Category: Large Structures]]
 [[Category: Gayen, A]]
 [[Category: Mukhopadhyay, C]]

2ks9

From Proteopedia

Revision as of 07:35, 29 April 2020

Solution conformation of substance P in water complexed with NK1R

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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