5et0

From Proteopedia

(Difference between revisions)

Revision as of 07:43, 29 April 2020

Crystal structure of Myo3b-ARB2 in complex with Espin1-AR

Structural highlights

5et0 is a 4 chain structure with sequence from Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5et1
Gene:	Espn (LK3 transgenic mice), Myo3b (LK3 transgenic mice)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ESPN_MOUSE] Multifunctional actin-bundling protein. Plays a major role in regulating the organization, dimensions, dynamics and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in variouS mechanosensory and chemosensory cells.^[1] ^[2] [MYO3B_MOUSE] Probable actin-based motor with a protein kinase activity.

Publication Abstract from PubMed

Class III myosins (Myo3) and actin-bundling protein Espin play critical roles in regulating the development and maintenance of stereocilia in vertebrate hair cells, and their defects cause hereditary hearing impairments. Myo3 interacts with Espin1 through its tail homology I motif (THDI), however it is not clear how Myo3 specifically acts through Espin1 to regulate the actin bundle assembly and stabilization. Here we discover that Myo3 THDI contains a pair of repeat sequences capable of independently and strongly binding to the ankyrin repeats of Espin1, revealing an unexpected Myo3-mediated cross-linking mechanism of Espin1. The structures of Myo3 in complex with Espin1 not only elucidate the mechanism of the binding, but also reveal a Myo3-induced release of Espin1 auto-inhibition mechanism. We also provide evidence that Myo3-mediated cross-linking can further promote actin fiber bundling activity of Espin1.

Myosin III-mediated cross-linking and stimulation of actin bundling activity of Espin.,Liu H, Li J, Raval MH, Yao N, Deng X, Lu Q, Nie S, Feng W, Wan J, Yengo CM, Liu W, Zhang M Elife. 2016 Jan 19;5. pii: e12856. doi: 10.7554/eLife.12856. PMID:26785147^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Loomis PA, Zheng L, Sekerkova G, Changyaleket B, Mugnaini E, Bartles JR. Espin cross-links cause the elongation of microvillus-type parallel actin bundles in vivo. J Cell Biol. 2003 Dec 8;163(5):1045-55. Epub 2003 Dec 1. PMID:14657236 doi:http://dx.doi.org/10.1083/jcb.200309093
↑ Sekerkova G, Zheng L, Loomis PA, Changyaleket B, Whitlon DS, Mugnaini E, Bartles JR. Espins are multifunctional actin cytoskeletal regulatory proteins in the microvilli of chemosensory and mechanosensory cells. J Neurosci. 2004 Jun 9;24(23):5445-56. PMID:15190118 doi:http://dx.doi.org/10.1523/JNEUROSCI.1279-04.2004
↑ Liu H, Li J, Raval MH, Yao N, Deng X, Lu Q, Nie S, Feng W, Wan J, Yengo CM, Liu W, Zhang M. Myosin III-mediated cross-linking and stimulation of actin bundling activity of Espin. Elife. 2016 Jan 19;5. pii: e12856. doi: 10.7554/eLife.12856. PMID:26785147 doi:http://dx.doi.org/10.7554/eLife.12856

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5et0"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Myo3b-ARB2 in complex with Espin1-AR==
-<StructureSection load='5et0' size='340' side='right' caption='[[5et0]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='5et0' size='340' side='right'caption='[[5et0]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5et0]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ET0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ET0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5et0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ET0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ET0 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5et1|5et1]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5et0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5et0 OCA], [http://pdbe.org/5et0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5et0 RCSB], [http://www.ebi.ac.uk/pdbsum/5et0 PDBsum]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Espn ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Myo3b ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5et0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5et0 OCA], [http://pdbe.org/5et0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5et0 RCSB], [http://www.ebi.ac.uk/pdbsum/5et0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5et0 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
+[[Category: Lk3 transgenic mice]]
 [[Category: Li, J]]
 [[Category: Liu, H]]

5et0

From Proteopedia

Revision as of 07:43, 29 April 2020

Crystal structure of Myo3b-ARB2 in complex with Espin1-AR

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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