5eun

From Proteopedia

(Difference between revisions)

Revision as of 07:45, 29 April 2020

The Crystal Structure of Human Kynurenine Aminotransferase II, PLP-bound form, at 1.83 A

Structural highlights

5eun is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Gene:	AADAT, KAT2 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[AADAT_HUMAN] Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).^[1]

Publication Abstract from PubMed

Kynurenine aminotransferase II (KAT-II) is a 47 kDa pyridoxal phosphate (PLP)-dependent enzyme, active as a homodimer, which catalyses the transamination of the amino acids kynurenine (KYN) and 3-hydroxykynurenine (3-HK) in the tryptophan pathway, and is responsible for producing metabolites that lead to kynurenic acid (KYNA), which is implicated in several neurological diseases such as schizophrenia. In order to fully describe the role of KAT-II in the pathobiology of schizophrenia and other brain disorders, the crystal structure of full-length PLP-form hKAT-II was determined at 1.83 A resolution, the highest available. The electron density of the active site reveals an aldimine linkage between PLP and Lys263, as well as the active site residues, which characterize the fold-type I PLP-dependent enzymes.

Structure of the PLP-Form of the Human Kynurenine Aminotransferase II in a Novel Spacegroup at 1.83 A Resolution.,Nematollahi A, Sun G, Harrop SJ, Hanrahan JR, Church WB Int J Mol Sci. 2016 Mar 25;17(4). pii: E446. doi: 10.3390/ijms17040446. PMID:27023527^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Han Q, Cai T, Tagle DA, Robinson H, Li J. Substrate specificity and structure of human aminoadipate aminotransferase/kynurenine aminotransferase II. Biosci Rep. 2008 Aug;28(4):205-15. PMID:18620547 doi:10.1042/BSR20080085
↑ Nematollahi A, Sun G, Harrop SJ, Hanrahan JR, Church WB. Structure of the PLP-Form of the Human Kynurenine Aminotransferase II in a Novel Spacegroup at 1.83 A Resolution. Int J Mol Sci. 2016 Mar 25;17(4). pii: E446. doi: 10.3390/ijms17040446. PMID:27023527 doi:http://dx.doi.org/10.3390/ijms17040446

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5eun"

@@ Line 1: / Line 1: @@
 ==The Crystal Structure of Human Kynurenine Aminotransferase II, PLP-bound form, at 1.83 A==
-<StructureSection load='5eun' size='340' side='right' caption='[[5eun]], [[Resolution|resolution]] 1.82&Aring;' scene=''>
+<StructureSection load='5eun' size='340' side='right'caption='[[5eun]], [[Resolution|resolution]] 1.82&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5eun]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EUN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EUN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5eun]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EUN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EUN FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=LLP:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>LLP</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5eun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eun OCA], [http://pdbe.org/5eun PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eun RCSB], [http://www.ebi.ac.uk/pdbsum/5eun PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eun ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AADAT, KAT2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5eun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eun OCA], [http://pdbe.org/5eun PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eun RCSB], [http://www.ebi.ac.uk/pdbsum/5eun PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eun ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 5eun" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Aminotransferase 3D structures|Aminotransferase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Church, W B]]
 [[Category: Hanrahan, J R]]

5eun

From Proteopedia

Revision as of 07:45, 29 April 2020

The Crystal Structure of Human Kynurenine Aminotransferase II, PLP-bound form, at 1.83 A

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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